FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7793452
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT430510.5523996298431042No Hit
GATGTATACAGAGATGAAGCATTAAACAACCGGTTCCAGATCAAAGGTGT241390.30973437701290774No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA238170.3056027033976728No Hit
CTAAAATAGATCTCTGGTCATACAACGCGGAGCTTCTTGTTGCCCTGGAG235260.30186879960253815No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA228490.29318201998292925No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG217650.2792729075639396No Hit
TCTAATAACAGTTTCTTTAGTAGATTGAATTGGTTGACCCACTTAAAATT210380.2699445637183625No Hit
GTTATTAGATCTCCTTTTGCAAGCAGAGCTTGTTCCATTTTGAGTGACTC206270.2646709057809043No Hit
GTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA198880.2551885865210949No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC198760.25503461110686254No Hit
ATCTGGGACAGGCCCCATATGTGATCCTGTTTACATTTTGAAATGGTTTG169810.21788804242330612No Hit
GTCCAATAGATGCTTATTCTGCTGGGGATATCCCTTACCCTGGGTCTAGA135370.17369709853861934No Hit
CTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGT134790.17295288403649628No Hit
CTGTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGC131070.1681796461952932No Hit
ACATTCTATGTCTGGTTTTCGCTCAAAAACTTCCCGGAAATGACAACAGC127910.1641249602871744No Hit
CTATTAACCATGAAGACTATCTTTGCTTTGAGCTACATTCTATGTCTGGT127420.1634962273457256No Hit
ATTTTAGTGTCCTCAACATATTTCTCGAGGTCCTGAATTCTCCCTTCTAC125740.1613405715464726No Hit
TCATAAGGGTAACAGTTGCTGTGGGCTTTGCTGCGTTCAACAAAAAGGTC123540.1585176889522127No Hit
CTATTGGACAATAGTAAAACCGGGAGACATACTTTTGATTAACAGCACAG122650.1573757046299894No Hit
CTATTTTAGTGTCCTCAACATATTTCTCGAGGTCCTGAATTCTCCCTTCT119880.15382143881812577No Hit
TCTCTGTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCA119190.15293608018628974No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT118100.15153747017367913No Hit
ACATACAATTGATCTAACTGACTCAGAAATGAACAAACTGTTTGAAAGAA116510.1494972959351004No Hit
CCCTTACCCTGGGTCTAGATCCGATATTCGGGATTACAGTTTGTTGGCTT114620.14707218316094076No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT113550.1456992357173689No Hit
ATTGTATGTTGGTTCTCCAGGGCAACAAGAAGCTCCGCGTTGTATGACCA113510.14564791057929144No Hit
TCTCAAAAGCACTCAAGCAGCAATCAACCAAATCAATGGGAAGCTGAATA110770.14213213862098592No Hit
TAGTAAAACCGGGAGACATACTTTTGATTAACAGCACAGGGAATCTAATT109650.14069503475481723No Hit
GTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGT109500.1405025654870268No Hit
GTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCTTT108890.13971985713134566No Hit
GTTTTACTATTGTCCAATAGATGCTTATTCTGCTGGGGATATCCCTTACC108560.13929642474220666No Hit
CTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAAGAAATGGGACCT108490.13920660575057114No Hit
CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCT108260.13891148620662577No Hit
ATGTGATCCTGTTTACATTTTGAAATGGTTTGTCATTGGGAATGCTTCCA104840.13452318690100357No Hit
ATCTATTAGTGTGCAGTTTTCTCCATCAAGGATCTGATGAGGACTGTCGC104560.1341639109344614No Hit
CATATGGGGCCTGTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCA104450.1340227668047484No Hit
CACTAAAATAGATCTCTGGTCATACAACGCGGAGCTTCTTGTTGCCCTGG104290.1338174662524386No Hit
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT102440.1314436786163564No Hit
GTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCAACAGGGATGCGA100410.12883892785892567No Hit
GCATTAATGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGC99830.12809471335680261No Hit
GTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATA98720.1266704407751533No Hit
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCG98420.12628550223957238No Hit
GGTATATCTTTCGCTCCGAGTCGGCATTTTGGGCCGCCGGGTTATTAGTA95620.12269274257415072No Hit
CCCTTATGATGTGCCGGATTATGCCTCCCTTAGGTCACTAGTTGCCTCAT92650.11888185107189984No Hit
ACTAAAGAAACTGTTATTAGATCTCCTTTTGCAAGCAGAGCTTGTTCCAT92320.11845841868276086No Hit
ATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG88160.11312060432270578No Hit
ATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAAT87410.11215825798375355No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG87220.11191446357788563No Hit
GTCCGTACCCGGGTGGTGAACTCCCCAAATGTACAATTTGTCAAAATTTT85720.10998977089998116No Hit
GGATCACATATGGGGCCTGTCCCAGATATGTTAAGCAAAACACTCTGAAA85200.10932254410497427No Hit
TTCTATGTCTGGTTTTCGCTCAAAAACTTCCCGGAAATGACAACAGCACG83880.10762881454841834No Hit
ATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTT83870.10761598326389898No Hit
ATACAGAGATGAAGCATTAAACAACCGGTTCCAGATCAAAGGTGTTGAGC83730.10743634528062788No Hit
TCTATTAGTGTGCAGTTTTCTCCATCAAGGATCTGATGAGGACTGTCGCA81580.10467761910896481No Hit
GGGTAAGGGATATCCCCAGCAGAATAAGCATCTATTGGACAATAGTAAAA81080.10403605488299664No Hit
ACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATA80650.10348430964866404No Hit
ATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGC80510.10330467166539295No Hit
TTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGT80250.1029710582678895No Hit
ATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTT80150.10284274542269588No Hit
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGA79630.10217551862768898No Hit
GATCTAACTGACTCAGAAATGAACAAACTGTTTGAAAGAACAAAGAAGCA79080.101469797979124No Hit
TTCTTTAGTAGATTGAATTGGTTGACCCACTTAAAATTCAAATACCCAGC78430.1006357644853654No Hit
CACTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAAT78210.10035347622593942No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA11050.058.405752
TTCGGAC11050.057.73323
TCGGACA11900.054.8242844
CTTTCGG12000.053.812351
GACATCT11950.052.774387
CGGACAT12550.051.4068375
CCGTTCC11400.050.264534145
GGACATC13950.046.2477266
GTCCAGA11800.037.5077551
CATGGTC214200.032.5893639
ATCATGG219500.032.0668687
TACATCA221000.031.980834
TATACAT221900.031.862162
ATACATC223500.031.6251343
GAGTGGC15400.029.1840526
AAGCCCG10050.028.869104145
AGAGTGG16000.028.542715
TATCCCG115750.027.9311285
TCCCGTG115300.027.7884797
CCGTGTT116400.027.5256989
Produced by FastQC (version 0.11.5)