FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7720001
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCTTCCG257410.33343259929629543No Hit
GAGCAAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCGTGGTC157700.20427458493852527No Hit
CTAGTGATCCGGCCATGGCACGTGGGTGCGTGGTCGCTCAACGGATAAAA148350.19216318754362854No Hit
TACTAGGGACGGCCCTTCTCAAATTTCCTGCGCGCACAGAGGATAGGGAC126870.1643393569508605No Hit
GGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACG118660.15370464330250735No Hit
GTTTAATTTTGAGGATGGTTATTTTTATCCAGCTAAGGAAAGCATCGATA92750.12014247148413582No Hit
TAGCCAACCAGCCATGCACCTGGCGGTACAACTGGCACACCAGAGGTTCG92180.11940412961086405No Hit
GATAAAAGGTACCCCGGGGATAACAGGCTGATCTTGCCCAAGAGTCCATA88480.11461138411769636No Hit
AAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCTTCCGAACGT86760.11238340513168327No Hit
GAACTAGTGATCCGGCCATGGCACGTGGGTGCGTGGTCGCTCAACGGATA86320.11181345701898225No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG80380.10411915749751846No Hit
ATATTGACCTGCTGCCAACATACAACCGACCTTATTTTCAGGGTCAATCT79180.1025647535537884No Hit
ATACTGACCTGCTGCCAACATACAACCAACCTTATTTTCAGGGTCAATCT77520.10041449476496182No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA221550.084.713841
GCAAAAG281950.066.33713
AAAAGCA288950.065.074125
AGCAAAA289600.064.6231842
CGAAAGC106900.056.3549653
CAAAAGC338300.055.539934
AAGCAGG378550.054.592767
GCGAAAG112150.053.4047242
AGTAGAA88900.051.3308681
GCAGGCT113850.047.6880769
GAGCGAA77450.047.210521
AGCGAAA129500.045.8821561
AGCAGGC158950.044.7372788
AAAGCAG523350.042.5075156
GGTCGAT21200.039.6964651
TAGTGCG20950.034.946077
ACAAGGC70950.034.7404483
CCTATGC42600.034.7159843
GTCCTAT44050.034.586221
AGTGCGC21100.034.3541038
Produced by FastQC (version 0.11.5)