FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences813809
Sequences flagged as poor quality0
Sequence length151
%GC60

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCTGCACGCGCTCACGCGCAATCTCGCGATTGAACTCGCCGATGCGGG86291.0603225081069392No Hit
AAGCAGGTCTGCACGCGCTCACGCGCAATCTCGCGATTGAACTCGCCGAT75740.9306852099202638No Hit
AGCGAAAGCAGGTCTGCACGCGCTCACGCGCAATCTCGCGATTGAACTCG63330.7781924259869331No Hit
GTCTTCGGGCGTACCGACGCGTCCGAGCGGATGAAACGAATCGAGCGCTT54760.6728851610144395No Hit
CAGGAAGACAATCGTGTTCGCAACGTCTTCGGGCGTACCGACGCGTCCGA54490.6695674292125057No Hit
AGACAATCGTGTTCGCAACGTCTTCGGGCGTACCGACGCGTCCGAGCGGA35660.43818635576652504No Hit
GAAGACAATCGTGTTCGCAACGTCTTCGGGCGTACCGACGCGTCCGAGCG34850.4282331603607235No Hit
AGCGAAAGCAGGAAGACAATCGTGTTCGCAACGTCTTCGGGCGTACCGAC34280.42122905988997417No Hit
TTCCAGGGCAGCCTCACCGGGATGGACAACCTGCGGTTCATCTGCCGCAT32780.4027972165458972No Hit
GAAAGCAGGTCTGCACGCGCTCACGCGCAATCTCGCGATTGAACTCGCCG32560.4000938795220991No Hit
AAAGCAGGGCGAGCGAGCGCACCACCCGCTGAATGCCGGTGCCTGCATCA30400.3735520251066282No Hit
GTCTGCACGCGCTCACGCGCAATCTCGCGATTGAACTCGCCGATGCGGGT30210.37121732494971177No Hit
AGCGAAAGCAGGGCGAGCGAGCGCACCACCCGCTGAATGCCGGTGCCTGC29150.3581921556532307No Hit
GCTTTATCTGGAACGCCTGATTCAGCCGAATACCCCCGACGTTGCAGTCG27090.33287909079403155No Hit
ATCGTGTTCGCAACGTCTTCGGGCGTACCGACGCGTCCGAGCGGATGAAA26180.32169710583195815No Hit
GCCCTGGAACGCACCGCCGAATGCGAGCGGCCACGACACGCTCATGCTGC25010.30732026802357804No Hit
TTACTGGGGCGATTGAGCTGGAGTTGGGCCGGCACGGCGCCAACTCCGGT23670.29085448796953584No Hit
GCAGGAAGACAATCGTGTTCGCAACGTCTTCGGGCGTACCGACGCGTCCG23030.2829902348093963No Hit
CTCACGCGCAATCTCGCGATTGAACTCGCCGATGCGGGTATCCGGGTGAA22340.27451158687112087No Hit
GGGCATGATCGCGTGGGGCGTTCTGTCGCTTTTGTTGGCGGGCCTGCGCG21400.26296096504216593No Hit
ATCGATGGCGATCGAATTTGACTGCTTCCTCATCGACGAAATCATTGCGG20500.25190185903571966No Hit
GACAATCGTGTTCGCAACGTCTTCGGGCGTACCGACGCGTCCGAGCGGAT18570.22818622059967386No Hit
GTATGCGTGCGCGCCTCGCGTTCGCGTTATCGATGGCGATCGAATTTGAC18500.22732606791028362No Hit
TCTATGGCGTCAATGTCAAAGATGCCGAACCGTTCGTACAAGAGTTCTCG18470.22695743104340207No Hit
GCACGCGCTCACGCGCAATCTCGCGATTGAACTCGCCGATGCGGGTATCC18450.22671167313214774No Hit
GAATAGATGCCCGGGATCGGACTGCAAACATACCGGAGTTGGCGCCGTGC18110.22253378864082357No Hit
CCCCAGTAACGCACGCTCAATAGTTGAATTCGGATGATCGAACTCAAGAA17220.21159756159000453No Hit
GGTATGCGTGCGCGCCTCGCGTTCGCGTTATCGATGGCGATCGAATTTGA16620.2042248242523737No Hit
CTCAAGAACATCGCCAAAGACTACTACACGCGACAGGGCCGACGCAGGGT16240.19955542393854087No Hit
GTATTTGCGCTGGGCATGATCGCGTGGGGCGTTCTGTCGCTTTTGTTGGC15990.19648345004786133No Hit
ATGATTGACACGTCGACGAGCAACTGGCGTTCCCGCGACGCGGGCGCTGA15950.19599193422535263No Hit
GTTCTTGAGTTCGATCATCCGAATTCAACTATTGAGCGTGCGTTACTGGG15920.19562329735847106No Hit
GATTGACACGTCGACGAGCAACTGGCGTTCCCGCGACGCGGGCGCTGATT15920.19562329735847106No Hit
GCGAAAGCAGGTCTGCACGCGCTCACGCGCAATCTCGCGATTGAACTCGC15140.18603873881955102No Hit
CACCACAACTGAGCCGCGGTTCATACGGCATCGCCGCGCGACATTTGTCG15140.18603873881955102No Hit
CTTTGACATTGACGCCATAGATGCGGCAGATGAACCGCAGGTTGTCCATC14960.18382691761826178No Hit
CGTCAAACATGCGCAACCGGCCTTGCACCAGCACGGCTGCCCGAGTGCAA14860.18259812806198997No Hit
AGCAGGAAGACAATCGTGTTCGCAACGTCTTCGGGCGTACCGACGCGTCC14840.18235237015073563No Hit
CATCTATGGCGTCAATGTCAAAGATGCCGAACCGTTCGTACAAGAGTTCT14610.1795261541713105No Hit
ATCTGGAACGCCTGATTCAGCCGAATACCCCCGACGTTGCAGTCGAACCC14410.17706857505876686No Hit
TGTTTGACGAGATCGAAGAGGCCTACGCGTTCTACCAGGAAAACAGCGCC14280.17547114863561353No Hit
AGCGAAAGCAGCTTTATCTGGAACGCCTGATTCAGCCGAATACCCCCGAC14140.173750843256833No Hit
ATGTCAAAGATGCCGAACCGTTCGTACAAGAGTTCTCGGAACTGGGCTAC14010.17215341683367963No Hit
GATGAGGGCTCGATCCGCGCGTCGTTCAAACAATTCGTGGTGGCACTTGG13880.1705559904105263No Hit
CTCAATAGTTGAATTCGGATGATCGAACTCAAGAACATCGCCAAAGACTA13760.16908144294300015No Hit
GGAAGACAATCGTGTTCGCAACGTCTTCGGGCGTACCGACGCGTCCGAGC13410.16478067949604883No Hit
ACTATTGAGCGTGCGTTACTGGGGCGATTGAGCTGGAGTTGGGCCGGCAC13340.16392052680665856No Hit
GTACCGACGCGTCCGAGCGGATGAAACGAATCGAGCGCTTTCATCGCACC13340.16392052680665856No Hit
CCATAGATGCGGCAGATGAACCGCAGGTTGTCCATCCCGGTGAGGCTGCC13190.16207734247225086No Hit
GTTCGCGGATGTACGCGGCATCGTGGCTGACGATGATGAGGGCTCGATCC13110.1610943108272334No Hit
CGCTCACGCGCAATCTCGCGATTGAACTCGCCGATGCGGGTATCCGGGTG13050.16035703709347035No Hit
CAATAGTTGAATTCGGATGATCGAACTCAAGAACATCGCCAAAGACTACT12920.158759610670317No Hit
AAATCATTGCGGTCGGCGACAGCCGGTTTCACGCCAAGTGCCACCACGAA12920.158759610670317No Hit
TTCCTGCGTCGCGCGGCGCGCGTCGCGCCCGTGCTGATCCTCTGCCTCGC12740.15654778946902773No Hit
GGTGGAATGAGCGCGTCAGTTGATGGCGCTGTTTTCCTGGTAGAACGCGT12620.15507324200150158No Hit
GGGCGAGCGAGCGCACCACCCGCTGAATGCCGGTGCCTGCATCATGTTGC12420.15261566288895798No Hit
TCCGCGAACATTGCACTCGGGCAGCCGTGCTGGTGCAAGGCCGGTTGCGC12360.1518783891551949No Hit
GATGTACGCGGCATCGTGGCTGACGATGATGAGGGCTCGATCCGCGCGTC12260.15064959959892307No Hit
ACAATCGTGTTCGCAACGTCTTCGGGCGTACCGACGCGTCCGAGCGGATG12200.14991232586516No Hit
GATGAAACGAATCGAGCGCTTTCATCGCACCTGCAATGTCTTCCTTCGCC12160.1494208100426513No Hit
TGTCAGGGGCATCGGTCGGGTGGAATGAGCGCGTCAGTTGATGGCGCTGT12150.1492979310870241No Hit
GCCTTGCACCAGCACGGCTGCCCGAGTGCAATGTTCGCGGATGTACGCGG12070.14831489944200665No Hit
GATTTCGTCGATGAGGAAGCAGTCAAATTCGATCGCCATCGATAACGCGA12050.14806914153075232No Hit
GGCAAGGACCGCTGACATGGCGCCGTGCCCTCTTTCCGACCCCGCATTCC11920.14647171510759896No Hit
ACCTGCGAGAGCCGGTCAAGAGCTATTCGGCAGGTATGCGTGCGCGCCTC11860.1457344413738359No Hit
GTACAAGAGTTCTCGGAACTGGGCTACTACCTGCGAGAGCCGGTCAAGAG11180.13737867239118762No Hit
GCATCATGTTGCGCAATGATTGACACGTCGACGAGCAACTGGCGTTCCCG11100.13639564074617017No Hit
TCTCGGAACTGGGCTACTACCTGCGAGAGCCGGTCAAGAGCTATTCGGCA11090.13627276179054298No Hit
CTCTTGTACGAACGGTTCGGCATCTTTGACATTGACGCCATAGATGCGGC10930.13430669850050808No Hit
GTCTTCCTTCGCCATGAAGCCTTCATAAATCGACGTCTGTACGATCGCCG10920.13418381954488093No Hit
GTCAAATTCGATCGCCATCGATAACGCGAACGCGAGGCGCGCACGCATAC10710.13160336147671015No Hit
GTTGACGCGCAGGTCGATCTTTTCGAGGACCCTGCGTCGGCCCTGTCGCG10680.1312347246098286No Hit
TCATACGGCATCGCCGCGCGACATTTGTCGTGGAATAGATGCCCGGGATC10660.13098896669857424No Hit
GGTGAACGCTGTCTCGCCGGCGATCGTACAGACGTCGATTTATGAAGGCT10430.1281627507191491No Hit
GGCTCGATCCGCGCGTCGTTCAAACAATTCGTGGTGGCACTTGGCGTGAA10420.1280398717635219No Hit
AGCGAAAGCAGGGCAAGGACCGCTGACATGGCGCCGTGCCCTCTTTCCGA10100.12410774518345213No Hit
ATTCGTGGTGGCACTTGGCGTGAAACCGGCTGTCGCCGACCGCAATGATT10070.12373910831657059No Hit
GACTACTACACGCGACAGGGCCGACGCAGGGTCCTCGAAAAGATCGACCT10040.12337047144968906No Hit
GAATGAGCGCGTCAGTTGATGGCGCTGTTTTCCTGGTAGAACGCGTAGGC9980.12263319771592597No Hit
AGCAAAAGCAGGGCGAGCGAGCGCACCACCCGCTGAATGCCGGTGCCTGC9860.12115865024839982No Hit
CGAAAGCAGGTCTGCACGCGCTCACGCGCAATCTCGCGATTGAACTCGCC9780.12017561860338238No Hit
CATCAGGCTCATCAGCGGTGCGGAACTCCCGAGTGCCGGCATGGTTCATC9750.11980698173650083No Hit
AAAGCAGGCCGACCATCATCGCCTGGTGCGCGAGGGTCATCACTTGTTCG9670.11882395009148339No Hit
CATTCCACCCGACCGATGCCCCTGACAAGGTTCAATCGCGCCGCACTCGC9670.11882395009148339No Hit
CCTATTCGCTGAGCGTGCAGACGATGCTGCTGCTCACGATGCTGTCGTTC9550.11734940262395721No Hit
GGTATCCGGGTGAACGCTGTCTCGCCGGCGATCGTACAGACGTCGATTTA9440.11599773411205823No Hit
GTTCAAACAATTCGTGGTGGCACTTGGCGTGAAACCGGCTGTCGCCGACC9370.11513758142266796No Hit
ATGCCGAACCGTTCGTACAAGAGTTCTCGGAACTGGGCTACTACCTGCGA9290.11415454977765052No Hit
GGCATGCCCCGCTGACCCGATACGCACGCCACGCAGCCGATCGGCTGCGC9240.11354015499951463No Hit
ATCGTACAGACGTCGATTTATGAAGGCTTCATGGCGAAGGAAGACATTGC9210.11317151813263308No Hit
GTTCATACGGCATCGCCGCGCGACATTTGTCGTGGAATAGATGCCCGGGA9210.11317151813263308No Hit
CAATTCGTGGTGGCACTTGGCGTGAAACCGGCTGTCGCCGACCGCAATGA9150.11243424439887001No Hit
GGGCTACTACCTGCGAGAGCCGGTCAAGAGCTATTCGGCAGGTATGCGTG9120.11206560753198846No Hit
GTGCTCGACCGCGCGTACAACGACGGCTACAAGCCGTTCTCCGACGGCTC9050.11120545484259821No Hit
CGATAACGCGAACGCGAGGCGCGCACGCATACCTGCCGAATAGCTCTTGA8910.10948514946381768No Hit
TAGTAGTCTTTGGCGATGTTCTTGAGTTCGATCATCCGAATTCAACTATT8900.1093622705081905No Hit
TCATTGCGGTCGGCGACAGCCGGTTTCACGCCAAGTGCCACCACGAATTG8870.10899363364130896No Hit
ACGATGATGAGGGCTCGATCCGCGCGTCGTTCAAACAATTCGTGGTGGCA8810.10825635990754587No Hit
GTTTTCCTGGTAGAACGCGTAGGCCTCTTCGATCTCGTCAAACATGCGCA8800.10813348095191869No Hit
TGCCGGCACCGTTCCTGCCGAGAATGCCGATTTTCTCGCCCCTGTTGACG8790.10801060199629152No Hit
CATCATCGTCAGCCACGATGCCGCGTACATCCGCGAACATTGCACTCGGG8770.10776484408503714No Hit
GTCAAGAGCTATTCGGCAGGTATGCGTGCGCGCCTCGCGTTCGCGTTATC8740.1073962072181556No Hit
TTTCCTGGTAGAACGCGTAGGCCTCTTCGATCTCGTCAAACATGCGCAAC8730.10727332826252843No Hit
GAACTGGGCTACTACCTGCGAGAGCCGGTCAAGAGCTATTCGGCAGGTAT8690.1067818124400197No Hit
GCGATGAACCATGCCGGCACTCGGGAGTTCCGCACCGCTGATGAGCCTGA8690.1067818124400197No Hit
CTTCGATCTCGTCAAACATGCGCAACCGGCCTTGCACCAGCACGGCTGCC8630.10604453870625664No Hit
GAATTTGACTGCTTCCTCATCGACGAAATCATTGCGGTCGGCGACAGCCG8610.10579878079500227No Hit
GGGCAAGGACCGCTGACATGGCGCCGTGCCCTCTTTCCGACCCCGCATTC8570.10530726497249354No Hit
ACCTGCGGTTCATCTGCCGCATCTATGGCGTCAATGTCAAAGATGCCGAA8530.10481574914998483No Hit
ATTCAGCCGAATACCCCCGACGTTGCAGTCGAACCCAAGCGCCTGAAGTC8500.10444711228310329No Hit
GTCGAGCACCGGCGACATCACGAAGAAGGTGAGGAACATCGCGAGGCCGA8430.10358695959371302No Hit
CTGCACGCGCTCACGCGCAATCTCGCGATTGAACTCGCCGATGCGGGTAT8390.1030954437712043No Hit
GATCGAGCCCTCATCATCGTCAGCCACGATGCCGCGTACATCCGCGAACA8370.10284968585994995No Hit
ATTGAACTCGCCGATGCGGGTATCCGGGTGAACGCTGTCTCGCCGGCGAT8300.10198953317055968No Hit
CAACTGACGCGCTCATTCCACCCGACCGATGCCCCTGACAAGGTTCAATC8280.10174377525930531No Hit
ATGATGATGCGCGTGAAGCTCGTCATCATCAGCAGCATCGCCGGCAGGAA8240.1012522594367966No Hit
GGGCATGCCAGTGCGTTGCGGCGCAGTGAGCGAGTGCGGCGCGATTGAAC8230.10112938048116941No Hit
TGTCGTGGCCGCTCGCATTCGGCGGTGCGTTCCAGGGCAGCCTCACCGGG8220.10100650152554222No Hit
CTTTCATCGCACCTGCAATGTCTTCCTTCGCCATGAAGCCTTCATAAATC8210.10088362256991505No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8170.10039210674740633No Hit
ATCCGGGTGAACGCTGTCTCGCCGGCGATCGTACAGACGTCGATTTATGA8150.10014634883615198No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCCAG100.007082198145.05766145
AAGGGAA100.0070939194144.977455
AGCGAAA22000.0130.929871
GCGAAAG26450.0109.109182
GAGCAAA2300.0104.10151
CGAAAGC28700.0100.78943
CCTATCA1250.098.614962
CCCTATC1400.088.103221
GAAAGCA32950.087.778464
TAGAAAC502.2686436E-886.997163
AGCAAAA4600.080.442071
CTATCAC1550.079.5135353
GAGCGAA3050.078.502771
TATCACC1600.077.019274
AAAGCAG44750.072.407735
ATCCGCC955.456968E-1268.711525145
TCACCGT1800.068.461576
AGAAACA651.3992394E-766.9126744
AGCAGGA8350.064.241517
GCAAAAG6050.063.5220382
Produced by FastQC (version 0.11.5)