FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2383753
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA43900.18416337598736113No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG33920.1422966221752002No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA31540.13231236625606763No Hit
GCAGAGGCCATGGAGGTTGCTAATCAGACTAGGCAGATGGTACATGCAAT31540.13231236625606763No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT31010.13008898153458012No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT30470.12782364615797023No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT30120.126355373228686No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA29070.12195055444083343No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT27690.11616136403394145No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA26430.11087558148851831No Hit
GTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATG24690.1035761674972197No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG24650.10340836487673011No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT24490.10273715439477162No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA24070.10097522687963056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA9150.0101.517651
CGAAAGC28850.080.66133
GCGAAAG29600.078.389062
GAGCAAA62550.071.467091
AGCGAAA35500.064.8012851
GCAAAAG92500.047.49383
AGTAGAA34550.046.8392031
CAAAAGC94650.046.5632634
AAGCAGG108150.046.2459757
TAGAAAC42900.045.8046042
CAATTCG7200.045.303469
GTAGAAA43500.045.2098161
AGCAAAA99550.044.50382
GAAAGCA56100.043.0270774
AAAAGCA113050.039.1769945
GCAGGGT28000.037.278289
AAAGCAG144050.037.03536
AGGTCGT5400.034.9004446
AGCAGGG59500.034.110848
GCAGGTA22400.033.9775969
Produced by FastQC (version 0.11.5)