FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5179828
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG228190.44053586335299166No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA133860.2584255693432292No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA113780.21965980337571053No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA100180.1934041053100605No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT87770.1694457808251548No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG83860.16189726763128043No Hit
GTACTATGTTCTTTTCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGG81350.1570515468853406No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT80370.1551595921717864No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC79630.15373097330644955No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC77640.14988914689831398No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT75930.14658787897976536No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT74630.1440781431352547No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA66840.12903903372853306No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT65510.12647138090299523No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC65260.1259887393944355No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG63090.12179941110013691No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT62080.11984953940555555No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT55870.1078607243329315No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT55750.10762905640882284No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC53500.10328528283178515No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA26600.0110.1820761
GAGCAAA177750.074.729131
GCGAAAG51550.066.107982
CGAAAGC53800.063.4675943
GCAGGTA49950.058.6285329
AGCAAAA225950.058.1153452
AAGCAGG271900.057.8519337
GCAGGGT116750.054.513089
GCAAAAG247250.053.4813043
AGCAGGG160800.053.1484838
CAAAAGC252300.052.9234434
GTAGAAA57550.049.4142231
AAAGCAG332600.048.6019976
AGGCTCG5350.047.453335145
AGCGAAA74750.045.419871
TAGAAAC62600.045.2884142
AGTAGAA43050.044.3193971
AAAAGCA304850.044.061655
CAATTCG8300.038.427119
AGCAGGT113000.036.7569358
Produced by FastQC (version 0.11.5)