FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK69.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK69.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5878407
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG226270.38491720631116555No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC211120.35914491800244525No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT195240.3321307966597073No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC188750.32109039064494854No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA184570.3139796206693412No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA130840.22257730708336457No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA121850.20728404821238133No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC120600.2051576217842691No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA114240.19433836411803404No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC112600.19154849264435075No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA105480.17943636770982344No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT93580.15919278811419488No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC93170.15849532024577406No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA92830.15791693225732756No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT91840.15623280252626265No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT88680.15085719651599488No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTT79930.1359722115192092No Hit
CATTAGCCTTTTCCCCTTTTGCAAGGTTACTCAGTTCATTGATGCTTAAT79800.13575106317068553No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC74520.1267690379383394No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG74370.12651386676696594No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT73430.12491479409302556No Hit
GTCATACTCTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA71320.12132538628237208No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGT71230.12117228357954801No Hit
CTCCTGTTCCACTTCAAACAGCAGTTGTAATGCTTGCATGAATGTTATTT69540.11829735504874025No Hit
GTATAGGAAACTTAAGAGAGAGATAACGTTCCATGGGGCCAAAGAAATAG69540.11829735504874025No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC68890.11719161330612188No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG68550.11661322531767535No Hit
CTTATCTCCTGTTCCACTTCAAACAGCAGTTGTAATGCTTGCATGAATGT68500.11652816826055086No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGTT67450.11474197006093656No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT66690.11344910279264434No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG66190.11259853222139943No Hit
CATTAATGCTGGTCCGTACCTTCTGTCTTCCTTACCTATAATGAGAAACC65570.11154382471305577No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT65430.1113056649531072No Hit
GTTCTTATCTCCTGTTCCACTTCAAACAGCAGTTGTAATGCTTGCATGAA65290.11106750519315862No Hit
TGATAACACAGTTCGAGTCTCTAAAAATCTACAGAGATTCGCTTGGAGAA64990.11055716285041169No Hit
ATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACAATTGC63100.10734200609110597No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA61740.10502845413731986No Hit
ATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTCAAC61200.10410983792037537No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG60530.10297007335490721No Hit
GGTGTGGAGACCATGGTCATTTTAAGTGCCTCATCAGATTCTTCTTTCAG60290.10256179948070965No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA30250.090.432331
GCAAAAG152250.086.468123
AGGCTCG6000.074.946655145
CAAAAGC207000.064.435694
CGAAAGC48600.063.6926163
GCAGGGT99150.061.8508349
AGTAGAA37400.060.3387761
GAGCAAA224400.059.9830861
AGCGAAA51750.059.1711241
AGCAAAA234550.056.887462
AGCAGGG144400.055.119228
GCGAAAG58000.053.136772
AGGGTGT50000.050.3081636
AAGCAGG324650.048.6980867
CGAGTCG3350.047.630997145
AGCAGGC18150.047.127388
GCAGGGG31550.045.9513789
AAAAGCA297300.045.108235
TAGAAAC78400.043.2876472
CAGTACG18300.042.383879
Produced by FastQC (version 0.11.5)