FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK68.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK68.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1434833
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA45920.3200372447525252No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG37570.26184231893188964No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT30520.21270768096356857No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC28650.19967480536062385No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA24740.1724242472817394No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC23810.16594265674123748No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT23680.16503662795600604No Hit
GTTCATTGATGCTTAATGCTGGTCCGTACCTTCTGTCTTCCTTACCTATA23010.16036709498596702No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC22750.15855503741550409No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC22540.15709145245474562No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA20940.14594032894420464No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA19360.13492859447754546No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT19050.13276806429737817No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG19040.13269836977543728No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC17270.12036243939190136No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT16720.11652924068515291No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA16580.11555351737798057No Hit
GTCATACTCTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA16460.11471718311469No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC16370.11408993241722207No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA15430.10753864735477926No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC15260.10635384048178428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA9100.098.067861
GCAAAAG45050.079.00253
AGGCTCG1900.076.36493145
AGTAGAA10000.068.201021
GAGCAAA55100.065.838731
CAAAAGC56400.064.00364
AGCAGGC6950.060.4877058
CGAAAGC14950.059.6373143
AGCAAAA60100.059.5974662
AGCGAAA15250.058.0049822
AGCAGGG32300.057.6706058
AAGCAGG79500.055.887837
GCAGGGG10600.054.7026069
GCAGGGT21850.054.070469
GCGAAAG18050.049.0069732
AAAAGCA80500.045.1124085
AAAGCAG103250.043.523676
GGTCGTT3200.043.035577
AGGGTGT12200.042.181546
CGTGTAG1557.421477E-1042.088655
Produced by FastQC (version 0.11.5)