FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3652747
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTATAGTGATTCGTTTCCCAAGATAAAAAGCCGCTATGTAAGCATCT68710.18810500699884225No Hit
GTATCAGTACACGGCACAGGCAGAAAACAGCCTGCAAATCACCCTACCGA64630.17693533113571785No Hit
CTATAGAGAAAGCTAAACAAATAGAAAAATAGAACCTATAAATAAAAACT46670.12776685601274876No Hit
GTATATGTGTATTATTACGGCAATGCTAAACGACACAAAGAGGCTCTTAC46390.12700030962998532No Hit
CACATATACTTTGCCGTCTTTTTCTGTTGAAATAGGCGAGAGCCCTTTGA43870.12010139218511437No Hit
GTATACTTATCGAGACGGCCTTTCTTACTAGTAACGAAGAGCGAAAGTTC41820.11448917759702493No Hit
ATCTTACAAAGTGCAAATTTCGTCTAGCCCCAAACGTTTGGAAGCTAAGC37600.10293622854251883No Hit
CTATAATACTCATTCTTGCAGTCGTGAATACACAGGCACAGGAACGACAA37410.10241607206850079No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG82500.046.762382
CGAAAGC83450.046.6485523
AGCGAAA91400.042.234381
GAAAGCA109050.036.557274
AAGCAGG130150.035.2530146
GAGCAAA73300.029.202411
AGCAGGG61700.028.8992567
AAAGCAG201350.026.3513055
AGCAGGC46650.025.3263537
AGCAAAA90950.022.9630032
TATAGTG64200.021.4516755
GCAGGGT22650.020.8008888
CAAAAGC101550.020.5565054
GCAGGGC19000.019.8374798
CTATAGT65100.019.8187274
GCAGGGG26700.019.5460038
TCTATAG71050.019.487653
TATTATA78850.019.1293642
GCAGGGA43500.018.995578
CACAGTC18150.018.7699855
Produced by FastQC (version 0.11.5)