FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3209747
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG135120.42096775851803897No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA99850.31108370846674205No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA73210.22808651273760827No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA65580.20431516876563793No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT61270.19088731915630733No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG55130.1717580856061241No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT54870.17094805291507398No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT51330.15991914627539178No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG49290.15356350516099868No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC48840.15216152550341194No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA48830.15213037039991No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT47730.14870330901469803No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC44620.13901407182559872No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC43870.13667743906295418No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT41780.1301660224310514No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT41530.12938714484350325No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT38370.11954213213689428No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT37880.11801553206529986No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTTGAAAATTTGCAGG32570.10147217210577657No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAC32420.10100484555324765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA20500.084.242551
GAGCAAA118700.075.129341
GCGAAAG34000.071.021422
AAGCAGG160900.064.828577
GCAGGGT59050.064.6932759
CGAAAGC38700.062.3766443
AGCAAAA142450.062.1551742
GTAGAAA36250.060.451541
GCAGGTA42350.056.8267869
AGCAGGG88100.056.6898088
GCAAAAG158650.055.7453273
AGTAGAA26300.055.1801221
CAAAAGC165350.054.0109334
AGGCTCG4700.050.930218145
AAAGCAG215800.049.4796376
AGCGAAA50900.047.6142651
TAGAAAC46750.046.6883962
AAAAGCA198750.045.1525155
GCTCGGA1801.8189894E-1244.299154
AGCAGGT81500.042.8697668
Produced by FastQC (version 0.11.5)