FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAACAGGCAGCGGAG13.7037037037037033No Hit
CTCGGAAAAGATGCCGGCACTTTAATTGAAGACCCAGATGAAAGCACATC13.7037037037037033No Hit
GTGTTAACGCTCACCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTT13.7037037037037033No Hit
GCACAGGGAATCTAATTGCTCCTAGGGGTTACTTCAAATTACGAAGTGGG13.7037037037037033No Hit
GCAGAATGGCATGCCATCCACACCAGCTGACTCTTGTGTGCTGGATTCTC13.7037037037037033No Hit
ATGTTGGAAAGCAAATTGTAGAAAAGATTCTGAAAGAAGAATCTGATGAG13.7037037037037033No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA13.7037037037037033No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCATTATTTT13.7037037037037033No Hit
GTCGCATATTTCACCTGTTGAGGAACTCTGAACCAGCTCAGTAGCATTAG13.7037037037037033No Hit
GGGCCATAAGGACCAGGAGTGGAGGAAACACTAATCAACAGAGGGCCTCC13.7037037037037033No Hit
TGCACATACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTAT13.7037037037037033No Hit
CACTTGATCCACCATTGCTCTTTGTGCAGCTGTTTGAAATTTTCCCTTAA13.7037037037037033No Hit
TACCTGGAAGAACACCCCAGCGCGGGGAAAGATCCCAAGAAAACTGGGGG13.7037037037037033No Hit
GTTGCACACTCTCTCATAAGCACTACTTGTTTTCCGTCCATTCTCACCTC13.7037037037037033No Hit
CACTAAAATAGATCTCTGGTCATACAACGCGGAGCTTCTTGTTGCCCTGG13.7037037037037033No Hit
GGTCTATTCCCACCAAGGAATATCCCTCTTTTTCGAAGTCGTACCCACTG13.7037037037037033No Hit
GAAGAACCCTTGTCAAGGTTACCAGCAACCTCCACATTGGCAAACCCTAA13.7037037037037033No Hit
TGGGAGACCCTCAGTGTGATGGCTTACAAAATAAGAAATGGGACCTTTTT13.7037037037037033No Hit
GAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAACGATT13.7037037037037033No Hit
GATAACTACTGTAACATTGCATTTCAAGCAATATGAATTCAACTCCCCCC13.7037037037037033No Hit
AGCCAATAGAGTCCTACAGACTTTCCCAATGGAACCTTCTTCCACTCCCT13.7037037037037033No Hit
TTTTTAATCTGTGTCTCACTTCTTCAATTAGCCATCTTATCTCTTCAAAC13.7037037037037033No Hit
CATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTTTTTAATCTGTGTC13.7037037037037033No Hit
CCTTATGATCATGTGAGTCAATACTCCTTAATAACCTCCCCTTCATATAT13.7037037037037033No Hit
GTTTCTTGACAATTCCTCAATAGTCATGTCAGTTATGTATCGCGAAGCAG13.7037037037037033No Hit
GAAACCTCCCATTTGAAAAGTCACCCATCATGGCAGCATTCACTGGAAAT13.7037037037037033No Hit
TATGTCTACTCTTCTGCGTATCTTGACTTGATTTCTAAAATGCACAGGGC13.7037037037037033No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)