FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4637640
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCTTCCG214820.4632097359864069No Hit
GAGCAAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCGTGGTC144810.3122493337128367No Hit
TACTAGGGACGGCCCTTCTCAAATTTCCTGCGCGCACAGAGGATAGGGAC111860.24120026565235766No Hit
CTAGTGATCCGGCCATGGCACGTGGGTGCGTGGTCGCTCAACGGATAAAA96490.20805840901838005No Hit
TAGCCAACCAGCCATGCACCTGGCGGTACAACTGGCACACCAGAGGTTCG74930.16156924642706205No Hit
GGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAGAACG63630.13720340518022098No Hit
GATAAAAGGTACCCCGGGGATAACAGGCTGATCTTGCCCAAGAGTCCATA62300.13433556722815915No Hit
GAGCGAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCTTCCG58920.1270473775454757No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA52480.11316100430391321No Hit
GCAAAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCTTCCGAA50800.10953847215394036No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49510.10675688496735408No Hit
AAAGCAGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCTTCCGAACGT49170.10602375346081196No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG48090.10369498279297229No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC47220.10181902864387922No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA178400.095.8903661
AGCAAAA199950.084.092452
GCAAAAG210800.079.748683
AAAAGCA232650.072.410745
CAAAAGC233350.072.255664
GAGCGAA59000.069.351051
AAGCAGG313850.064.186117
AGCAGGC110150.063.438538
GCAGGCT89500.059.609489
AAAGCAG365900.056.8589446
AGTAGAA50950.053.96611
GCGAAAG75950.053.1643833
AGCGAAA77600.051.9506232
CGAAAGC82650.048.5888754
ATTTCCG10900.046.582977145
AGCAGGG111950.044.4181638
CGTAGGA8050.038.746124145
AGCAGGA138100.038.684278
CGTCGAC2550.036.9553957
GTAGAAA90300.036.715921
Produced by FastQC (version 0.11.5)