FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6107061
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG62620.10253704687082706No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG60250.080.513862
AGCGAAA61850.078.5875551
CGAAAGC66500.073.037273
GAGCGAA26350.061.671721
GAGCAAA143250.054.137941
GCAAAAG179250.042.3831753
CAAAAGC182200.041.616694
AGCAAAA207900.036.7959142
AAAAGCA251350.030.656635
AAAGCAG301050.030.6279896
AAGCAGG291450.029.4976277
GAAAGCA184500.027.1104664
TGACGCG3555.456968E-1226.562105145
ACGACGC8450.020.587658
CGACGAA5002.5465852E-1120.309793145
TATACCG4102.1758751E-819.4481755
TACGACG9200.018.9099277
AGCAGGG202600.018.6759768
GTATATA63950.016.903021
AGCAGGC109400.015.7030238
Produced by FastQC (version 0.11.5)