FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7671657
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG165690.21597680918216236No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG143520.18707822834102203No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA107250.13980030650483982No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA102900.13413008428296522No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC96810.12619177317234073No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC92720.12086046078441724No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG85550.11151437036353423No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG85520.11147526538269374No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG84860.11061495580420241No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC84470.11010659105327571No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT80210.10455368377392264No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT77790.10139921531945445No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG91850.083.607622
GAGCGAA35950.079.919871
CGAAAGC99650.077.1109543
AGCGAAA100050.076.360671
GAGCAAA231500.063.0259781
CAAAAGC281400.051.2099464
GCAAAAG312600.046.1246723
AAGCAGG376550.043.563337
AGCAAAA345650.041.9797252
AAAAGCA355300.040.9870575
AAAGCAG432750.039.8659326
GAAAGCA205400.038.466984
AGTAGAA102800.036.065031
TAGAAAC121000.035.778162
GGTCGTT36900.035.5558787
CAATTCG66750.034.859079
GTAGAAA131400.034.344211
AAGGTCG54200.033.5688489
AGCAGGT165100.032.225928
AGGTCGT46800.029.1188356
Produced by FastQC (version 0.11.5)