FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6999373
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG77180.11026701963161556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG81350.093.415342
CGAAAGC87700.087.1245653
AGCGAAA90750.083.226911
GAGCGAA25750.068.186411
GAGCAAA134150.048.188861
GAAAGCA189600.041.7881854
AAGCAGG322150.033.0316546
AAAGCAG346250.032.8890955
CTCGATA29950.032.6737759
CAAAAGC202250.032.113764
GCAAAAG205700.031.6124213
AGCAAAA219650.029.2493972
CCTCGAT36850.026.7524648
GGCCTCG39100.025.583786
AGCAGGG217600.025.5171557
CCGACGC15650.024.564234145
AAAAGCA272400.024.3489075
GCCTCGA44450.022.6675957
TATACGC4205.2750693E-1122.436579
GACGACG4952.1827873E-1120.51467145
Produced by FastQC (version 0.11.5)