FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6157095
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC102250.080.397073
AGCGAAA108850.074.651141
GCGAAAG147900.055.3037832
GAGCAAA148850.043.2336241
GCAAAAG162500.039.212633
GAAAGCA225900.037.9292344
GAGCGAA65800.035.283441
AAGCAGG296050.035.110976
AGCAAAA188450.033.7071382
CAAAAGC196600.032.7786524
AAAGCAG411600.029.3753415
AGCAGGG172750.026.3929427
GTATAGA55300.023.2217521
AAAAGCA291850.022.6266865
AGCAGGC105000.020.2961317
TCTATAC53200.018.8043333
GCAGGGG79200.017.9383538
GGTCGAT6900.017.8589137
GTGACTG57400.017.3007099
GTGCGTT19800.017.2063169
Produced by FastQC (version 0.11.5)