FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAGAAATGGCCAACAGAGGCCTATGGGATTCCTTTCGTCAGTCCGAA25.263157894736842No Hit
CTACTGTACAAGTTCCATTGATACAAACGCATTCTGACTCCTGGGTCCTG12.631578947368421No Hit
ATGGCACTTCTTAGTAACATAAGGACACAGTATTTCTCCCATTTATGTGG12.631578947368421No Hit
GAGGCAATACTCTCGGTCTAGACATCAAAGCAGCCACCCATGTTGGAAAG12.631578947368421No Hit
ATCTAAAAACTTCTATGGTGTTGGCTAATGCAGTTGCTGCCGGTTGATTT12.631578947368421No Hit
TTCCTCAATAGTCATGTCAGTTATGTATCGCGAAGCAGGTGTGGAGACCA12.631578947368421No Hit
CTTCAAATGAGAACATGGATAATATGGGATCGAGCACTCTTGAACTGAGA12.631578947368421No Hit
AATCAACAGAGGGCCTCCGCAGGCCAAACCAGTGTGCAACCTACGTTTTC12.631578947368421No Hit
GCCTCTGCCCTTAACATGACTGTAGCCATGTTTATTGCATCTTATGCAGG12.631578947368421No Hit
AGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTCAACCC12.631578947368421No Hit
GGTCTGCAAATTTTCAAGAAGATCATCTCTTAAACCAGTACTGGAACTAG12.631578947368421No Hit
GAAGTGGCATTTGGCCTGGTATGTGCAACATGTGAGCAGATTGCTGACTC12.631578947368421No Hit
ATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCT12.631578947368421No Hit
TGCCATTCTGCTGCATTTGAAGATTTAAGATTGTTAAGCTTCATCAGAGG12.631578947368421No Hit
AGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCA12.631578947368421No Hit
TTTTCCATCTACTCTCCTGTATATGGGCCCCCCAGTTTTCTTGGGATCTT12.631578947368421No Hit
CTTGCAGAAAAAGCAATGAAAGAGTATGGGGAGGATCTGAAAATTGAAAC12.631578947368421No Hit
ACTCAAAACGGAACAAGCTCTGCTTGCATAAGGAGATCTAAAAACAGTTT12.631578947368421No Hit
AGCAGTAATGAGAATGGGGGATCTCCACTTACTCCAAAACAGAAACGGGA12.631578947368421No Hit
CGGTGAGCGTGAACACAAACCCCAAAATCCCCTTAGTCAGAGGTGACAGA12.631578947368421No Hit
TTGTCCATGTTATTTGGGTCTCCATTCCCATTGAGGGCATTTTGGACAAA12.631578947368421No Hit
GGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCT12.631578947368421No Hit
GGTCCATACGCACAGGCAGGTAGGCAAGATTTGTGAGCAACTGATCCTCT12.631578947368421No Hit
CCCGCGATTGCGCCAAATATGCCTCTAGTTTGTTTCTCTGGTACATTTCG12.631578947368421No Hit
GTGGAGCCCATGGAGCTCAAGGGAGAGAGGGAATTGGAACACCAGGTTTC12.631578947368421No Hit
GGCTATTGACTGTTCGTCTCTTCCACTCACTATGAGTTGAACCAATCTTC12.631578947368421No Hit
AATATGTTGAGGACACTAAAATAGATCTCTGGTCATACAACGCGGAGCTT12.631578947368421No Hit
GCATTGATATGTTTGAAAAATTCCCCAGGTGATTCTAATAGGCAGCCAGG12.631578947368421No Hit
CTTGAATCGTTGCATCTGCACCCCCATTCGTTTCTGATAGGTCTGCATAT12.631578947368421No Hit
ATTCTATTAACCATGAAGACTATCATTGCTTTGAGCTACATTCTATGTCT12.631578947368421No Hit
AAACAAGGCATTTTTTCATGAAGGATAAGCTAAATTCATTATTTTTGCCG12.631578947368421No Hit
GCATTTGGCCTGGTATGTGCAACATGTGAGCAGATTGCTGACTCCCAGCA12.631578947368421No Hit
TCAGAGAGCACATTCTGGGATCCATTCCAGTTCGAACAAGAGCTCTTGTC12.631578947368421No Hit
TCTGGAGTCACTTAGTCTAGGTGGCCAAATAGGTTGACTAAGTCTTTTTC12.631578947368421No Hit
GTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCCGGAAACAAGT12.631578947368421No Hit
CTATTGAATACTGACTTAGCCAATAGAGTCCTACAGCCTTTCCCAATGGA12.631578947368421No Hit
CATTCCTTCTTCTCATGCCCAGTGCGGAGAACTTTTGAAACTTTCATAAT12.631578947368421No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)