FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4875604
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA83790.1718556306049466No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC55000.11280653637990289No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG53560.1098530561546836No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53050.10880703190825178No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT52860.10841733660075757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA25250.089.933421
AGCGAAA49250.070.561441
GAGCAAA159750.064.034851
CGAAAGC55650.062.6577723
GCGAAAG58100.060.0340462
GCAAAAG169800.059.257983
CAAAAGC191200.053.7206654
AGCAAAA189150.053.5573122
AAGCAGG249900.047.1938257
AAAAGCA234650.043.865925
AAAGCAG309550.039.5518846
AGCAGGG132150.038.7254948
CAGTACG8950.033.206289
CGAGTCG1851.6382546E-731.367876145
AGTAGAA52650.031.1421241
GCAGGGT72850.031.0444879
TATCGTG6350.030.833212
AGGCTCG7300.030.803902145
TAGAAAC69000.029.4264352
ATATCGT6450.029.2450121
Produced by FastQC (version 0.11.5)