FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8416570
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG152730.18146347027351997No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA123970.1472927807883734No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA109050.1295658445186103No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG96270.11438151170845132No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT91710.10896362770107063No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT89220.10600517788125091No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA39050.081.9279561
AGCGAAA76700.072.45161
GAGCAAA227850.071.766111
GCGAAAG78350.071.0776142
CGAAAGC83150.067.481723
AGCAAAA318000.050.598872
CAAAAGC327900.049.0781254
AAGCAGG396250.047.6908767
GCAAAAG349250.046.1227343
AAAGCAG463550.041.5021366
AAAAGCA409750.039.6804355
AGCAGGG233150.038.2413528
GTAGAAA108400.035.0684471
TAGAAAC115650.031.2871152
CAATTCG24650.030.5831729
GCAGGTA70550.029.4883849
CGTGTAG11650.028.6218725
GCAGGGT160200.028.0992839
TCGTGTA12150.027.444674
AGTAGAA102300.026.5931761
Produced by FastQC (version 0.11.5)