FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2653335
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA38650.079.788331
GAGCGAA19500.079.630441
GAGCAAA76750.076.011211
GCGAAAG44200.070.546562
CGAAAGC46400.067.497713
CAAAAGC100700.057.083384
AGCAAAA104000.055.50212
GCAAAAG105300.054.4592173
AAAAGCA127700.045.6376155
AAAGCAG171150.042.0985646
AAGCAGG180350.038.785467
GCTATCG900.00363292332.2156648
GAAAGCA105350.030.9630664
TAGAAAC47300.030.5085542
GTAGAAA54500.027.6927971
AGTAGAA43500.027.1893181
TCGTCGG1100.0096449426.380144145
CCGACGC5050.025.857765145
AGCAGGC56200.025.2795528
AGCAGGA82050.024.2059218
Produced by FastQC (version 0.11.5)