FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6086719
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA214450.3523244624895613No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG198420.32598843482013873No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT189210.31085713008929766No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC181630.29840378699920267No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC134280.22061146571740867No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA121750.2000256624299561No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA113530.1865208497385866No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC113530.1865208497385866No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC107000.17579257396308257No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA105170.17278602807193824No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA104140.17109381918238709No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC100690.16542574086301667No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT99340.16320779717282824No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT92010.1511651843957311No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT89970.1478136250416686No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTT77200.12683352065373807No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC76220.12522345782678648No Hit
TCCTTACTCTGGGTCTAGATCCGATATTCGGGATTGCAGTTTGTTGGCTT75750.12445128483835051No Hit
TCCTAGTTCCAGTACTGGTTTAAGAGATGATCTTCTTGAAAATTTGCAGA70400.11566165614019638No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT70300.11549736401499724No Hit
GTATACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCAGGCAT70130.1152180674021587No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG68830.11308226977456985No Hit
GTATAGGAAACTTAAGAGAGAGATAACGTTCCATGGGGCCAAAGAAATAG67370.11068360474666236No Hit
GAATCACAGTCTCTACCAAAAGAAGCCAACAAACTGCAATCCCGAATATC67100.1102400160086247No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG65840.10816993523111548No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT64000.10514696012745126No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGT63670.10460479611429409No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA63610.1045062208391746No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG63270.10394762761349752No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG61440.1009410817223532No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA61390.10085893565975362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCTCG7750.091.72026145
GAGCGAA29350.081.812851
GCAAAAG146150.081.7971043
GCAGGGG27050.075.568519
GCAGGGT70150.074.91519
AGTAGAA29300.071.3060761
CGAAAGC42150.064.8425143
AGCAGGG117000.062.821918
AGGGTGT49900.060.7229166
CAAAAGC203800.060.255024
GAGCAAA216000.056.4567151
AGCGAAA49000.055.5183871
AGCAGGC11800.054.0579648
CGAGTCG2350.052.47132145
AAGCAGG282500.051.395927
TAGAAAC61850.050.41162
AGCAAAA243300.050.1882132
ACAAGGG68600.049.880873
AAGGGTG62650.049.1750375
CAAGGGT69900.049.1569754
Produced by FastQC (version 0.11.5)