FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7966943
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA244380.3067424983459779No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC133880.16804438038529962No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT124280.15599458914165698No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA116680.14645517107377323No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG112270.14091979822122488No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT111770.14029220492728517No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT104400.13104147977461367No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC103660.1301126416995829No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC96300.1208744684127902No Hit
TCCTTACTCTGGGTCTAGATCCGATATTCGGGATTACAGCTTGTTGGCTT96060.12057322363169913No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG93450.1172971866373338No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG88020.11048152346514843No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA87990.11044386786751204No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG86590.10868660664448083No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG86080.10804646148466229No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT82430.10346503043890236No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG80920.1015696986912044No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA80870.10150693936181042No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG80490.10102996845841622No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG80470.10100486472665864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA43000.078.28111
GCAAAAG222850.067.760383
AGTAGAA50150.061.1894841
CGAAAGC64000.057.541743
AGGCTCG10400.056.487186145
AAGCAGG326650.056.2346657
CAAAAGC280450.054.7974784
AGCGAAA67800.053.606251
GAGCAAA291350.053.285141
AGCAGGT159500.049.447348
AGCAAAA321750.047.664162
GCGAAAG79050.046.230522
AAAGCAG453850.040.633826
AGCAGGG159800.039.42018
GCAGGGG71500.039.0327729
AGGGTGT41600.036.768176
AAAAGCA424200.036.569865
TAGAAAC98600.036.5493472
GCAGGTA169250.035.2917569
CAGTACG32300.035.010389
Produced by FastQC (version 0.11.5)