FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7161038
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCCCAAGCAGAAAATGGAAGGACCTCTTTGCATCAGAATGGACCAGGC422580.590109981262493No Hit
GTACAAATTGCTTCAAATGAGAACATGGATAATATGGGATCGAGCACTCT365230.5100238261548117No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC247860.3461230061898848No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA202640.28297573619913763No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT196490.2743875957647481No Hit
GGAGTGGAGGAAACACTAATCAACAGAGGGCCTCCGCAGGCCAAACCAGT194850.2720974249822442No Hit
GAAAATGGAAGGACCTCTTTGCATCAGAATGGACCAGGCAATCATGGAGA141760.1979601281266766No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC140560.19628439340777132No Hit
GTCATACTCTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA139770.19518120138449202No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT132740.18536418882290528No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTT131650.18384206311989967No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT130000.1815379278814049No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT129660.1810631363777151No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA127500.17804681388368557No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG127340.17782338258783154No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA118040.16483643851631566No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC117770.16445939820456196No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA115720.16159668472643213No Hit
CTCCTGTTCCACTTCAAACAGCAGTTGTAATGCTTGCATGAATGTTATTT114050.1592646205759556No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC110410.15418155859527627No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT103280.14422490147378075No Hit
TGATAACACAGTTCGAGTCTCTAAAAATCTACAGAGATTCGCTTGGAGAA102250.1427865625067204No Hit
GCAGTTAAACTGTATAGGAAACTTAAGAGAGAGATAACGTTCCATGGGGC101100.1411806500677695No Hit
TCTCATTACTGCTTCTCCAAGCGAATCTCTGTAGATTTTTAGAGACTCGA98310.13728456684631474No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG96640.13495250269583822No Hit
GTTCTTATCTCCTGTTCCACTTCAAACAGCAGTTGTAATGCTTGCATGAA95700.13363984383269575No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG91740.12810991926030835No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA90710.126671580293248No Hit
GGATCAAGTGAACAGGCAGCGGAGGCCATGGAGATTGCTAGTCAGGCCAG90370.12619678878955817No Hit
CATTACTGCTTCTCCAAGCGAATCTCTGTAGATTTTTAGAGACTCGAACT90030.12572199728586833No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG89790.12538685034208727No Hit
TTTTTAATCTGTGTCTCACTTCTTCAATTAGCCATCTTATCTCTTCAAAC89430.12488412992641569No Hit
CTTATCTCCTGTTCCACTTCAAACAGCAGTTGTAATGCTTGCATGAATGT87860.12269171033584797No Hit
TCATTACTCATATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTC87390.12203538090427672No Hit
GTTTTTAATCTGTGTCTCACTTCTTCAATTAGCCATCTTATCTCTTCAAA83220.11621220275608089No Hit
TCCTAGTTCCAGTACTGGTTTAAGAGATGATCTTCTTGAAAATTTGCAGA81210.11340534710191455No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGT77980.10889482781686118No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG76070.10622761672260363No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT75220.10504063796337906No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC75180.10498478013941555No Hit
TTGTCATACTCTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACT74620.10420277060392642No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT72480.10121437702187869No Hit
ATATACAGGAGAGTAGATGGAAAATGGATGAGGGAACTCGTCCTTTATGA72100.10068372769422534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC61750.075.250473
AGCGAAA63950.072.493221
GAGCGAA25950.067.9369351
GCGAAAG74750.062.176462
AGTAGAA55800.055.777641
GCAAAAG107900.055.0238043
TAGAAAC88500.052.925922
AGGGTGT82100.050.4157186
AAATTGC125750.049.6903765
TACAAAT135050.047.2462432
ACAAGGG117550.044.833223
GCAGGGT62750.044.2437559
CAGTACG15800.044.0434279
CAAGGGT119250.043.098674
CGAGTCG5100.042.66332145
AAGGGTG102750.041.553395
CCCAAGC170750.039.3121074
GCCCAAG172800.038.8468133
CAAAAGC155800.038.5711984
GGTGTTT110700.037.521228
Produced by FastQC (version 0.11.5)