FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGAGAGAATGTGCAACATTCTTAAAGGAAAATTTCAAACAGCTGCACA22.7027027027027026No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC22.7027027027027026No Hit
TGAAAGCCCTTAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTG22.7027027027027026No Hit
GTCAACAGAACACATCAATATTCAGAGAGAGGGAAGTGGACGACAAATAC11.3513513513513513No Hit
CTGGTACATTTCGCATCCCTGTTGCCAATTTCAGAGTGTTTTGCTTAACA11.3513513513513513No Hit
CCACACCTGCTTCGCGATACATAACTGACATGGCTATTGAGGAATTGTCA11.3513513513513513No Hit
GTTTGGATCCTAACAATGAGGAAGGTGGTCATGGGGTGAAAGGCTGGGCC11.3513513513513513No Hit
NCTTGGTTGTTTGGGGGGGAGTTGAATTCATATTGCTTGAAATGCAATGT11.3513513513513513No Hit
GGCAACTAGTGACCTAAGGGAGGCATAATCCGGCACATCATAAGGGTAAC11.3513513513513513No Hit
GAACAGGAACAGGGTACACCATGGACACAGTCAACAGAACACATCAATAT11.3513513513513513No Hit
ATTTAACAAATTGTACATTTGGGGGGTTCACCACCCGGGTACGGACAAAG11.3513513513513513No Hit
AAATAAGATCCTTCATTACTCATATCAAAAGAGGGCACGATCGGGTTCGT11.3513513513513513No Hit
GACATAATGGATTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCT11.3513513513513513No Hit
CTTTAGTTCTGCACTTGCTTATCATGGGAATTAGTTGAAAATCGTCCATT11.3513513513513513No Hit
CCATTCTGATGCAAAGAGGTCCTTCCATTTTCTGCTTGGGCATTAGCATG11.3513513513513513No Hit
ACTTGGTTGTTTGGGGGGGAGTTGAATTCATATTGCTTGAAATGCAATGT11.3513513513513513No Hit
ATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCT11.3513513513513513No Hit
ATGCCCAAGCAGTAAATGGAAGGCCCTGGTTGCATCCGGGTGGAGCAGGC11.3513513513513513No Hit
GGTCCAAATTCCTCCTGATGGATGCTTTAAAATTGAGCATTGAAGACCCT11.3513513513513513No Hit
GAAGTTACTAATGCTACTGAGCTGGTTCAGAGTTCCTCAACAGGTGAAAT11.3513513513513513No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCGT11.3513513513513513No Hit
CAAGATGTAATTATGGAAGTTGTTTTTCCCAATGAAGTGGGAGCCAGGAT11.3513513513513513No Hit
ATATTCGGGATTGCAGTTTGTTGGCTTCTTTTGGTAGAGACTGTGATTCT11.3513513513513513No Hit
AATACAAGCAGGAGTGGATAGATTCTACAGGACCTGCAAGTTAGTGGGAA11.3513513513513513No Hit
CATATTTCTCAAGGTCCTGAATTCTCCCTTCGACTTCTGAGAATTCTTTT11.3513513513513513No Hit
GTTTCATGTATTCAGATTTACATTTCATCAATGAACAAGGCGAATCAATA11.3513513513513513No Hit
GAGATAACGTTCCATGGGGCCAAAGAAATAGCTCTCAGTTATTCTGCTGG11.3513513513513513No Hit
ATAAAGCTTCTCCCCTTTGCAGCTGCTCCACCAAAGCAAAGCAGAATGCA11.3513513513513513No Hit
ACACTACTGGAGCTGGGAAACCGAAGTTTCTACCAGATTTGTATGATTAC11.3513513513513513No Hit
CTCTTAAACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC11.3513513513513513No Hit
GTGTGTCACATGGTGAAATTGCCCAGGTATGGGTTGAAAAGTTTTCCCAA11.3513513513513513No Hit
CTGCTGGAGCTGTCGTTTTTTCTGGGTGTGTCTCCAACAAGTCCTGAACA11.3513513513513513No Hit
GGCTAATAGATTTCCTCAAGGATGTGATGGAATCAATGGATAAAGAGGAA11.3513513513513513No Hit
CTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGGACCAGGAGTGGAGGA11.3513513513513513No Hit
GTACCCGCTTCTCAGTTCAAGAGTGCTCGATCCCATATTATCCATGTTCT11.3513513513513513No Hit
TATGAGAGAATGTGCAACATTCATAAAGGAAAATTTCAAACAGCTGCACA11.3513513513513513No Hit
CACTCACTGAGTGACATCAAAATCATGGCGTCCCAAGGCACCAAACGGTC11.3513513513513513No Hit
AGATCGAAGACCTCATATTTTTAGCAAGATCTGCATTGATATTGAGAGGA11.3513513513513513No Hit
CTGTCCTCCTTGTAATCATACAAATCTGGTAGAAACTTCGGTTTCCCAGC11.3513513513513513No Hit
TTTGATGTCTAGACCGAGAGTATTGCCTCTTCCCCTTAGGGACCTCTGAT11.3513513513513513No Hit
GTTGAACGCGGCAAAGCCTACAGCAACTGTTACCCTTATGATGTGCCGGA11.3513513513513513No Hit
AAACAAGGGTGTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAA11.3513513513513513No Hit
CATATAAGAACATTGGCCTTGAAATTTGGCCTATGGCACTTCTTAGTAAC11.3513513513513513No Hit
CCCTCTCAAGACGGCGGACTACACTCCGGATGTGCTTTCATCTGGGTCTT11.3513513513513513No Hit
GCATCATTGAGCCCTGGAATGATGATGGGCATGTTCAACATGCTAAGTAC11.3513513513513513No Hit
CTAAAAACAGTTTCTTTAGTAGATTGAATTGGTTGACCCACTTAAACTTC11.3513513513513513No Hit
GTACAAGTTCCATTGATACAAACGCATTCTGACTCCTGGGTCCTGAGGAT11.3513513513513513No Hit
GGGCCTCCGCAGGCCAAACCAGTGTGCAACCTACGTTTTCTGTACAAAGA11.3513513513513513No Hit
GTTTATCATGTTGTTCTTTATCACTGTTACTCCAATGCTCATATCAGCTG11.3513513513513513No Hit
CCCATTGGCAAATGCAATTCTGGATGCATCACTCCAAATGGAAGCATTCC11.3513513513513513No Hit
CACAATTAACAATAACTAAAGAGAAAAAAGAAGAACTCCGAGATTGCAAA11.3513513513513513No Hit
GATAATAACGATTGGCTCTGTTTCTCTCACCATTTCCACAATATGCTTCT11.3513513513513513No Hit
CACTGAGGGTCTCCCAATAGAGCATCTATTAGTGTGCAGTTTTTTCCATC11.3513513513513513No Hit
GAATAGACCCTTTCCAACTACTTAAAAATAGCCAAATATACAGCTTAATA11.3513513513513513No Hit
ATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCTCTCTATA11.3513513513513513No Hit
CACTCACTGAGTGACATCAAAATCATGGCGTCCCAAGGCCCCAAACGGTC11.3513513513513513No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT11.3513513513513513No Hit
GACCAAAGATGCAGAGAGAGGCAAATTAAAAAGCAGGGCTATTGCCACGC11.3513513513513513No Hit
TCATATAGGAGACCGATTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC11.3513513513513513No Hit
ACCAAAAGAAGCCAACAAACTGCAATCCCGAATATCGGATCTAGACCCAG11.3513513513513513No Hit
CTAAACAACGTACATTCAAATAACACAGTACGTGATAGGACCCCTTATCG11.3513513513513513No Hit
CTTAAACCAGTACTGGAACTAGGATGCGTCCCAATGGCTCTCATTGCCTG11.3513513513513513No Hit
AGGTAAGGAAGACAGAAGGTACGGACCAGCATTAAGCATCAATGAACTGA11.3513513513513513No Hit
GGACTGTCGCATATTTCACCTGTTGAGGAACTCTGAACCAGCTCAGTAGC11.3513513513513513No Hit
GCTCTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAA11.3513513513513513No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT11.3513513513513513No Hit
TTCTTTAGTAGATTGAATTGGTTGACCCACTTAAACTTCAAATACCCAGC11.3513513513513513No Hit
AATATGTTGAGGACACTAAAATAGATCTCTGGTCATACAACGCGGAGCTT11.3513513513513513No Hit
GGTGAGAATGGACGGAAAACAAGAAGTGCTTATGAGAGAATGTGCAACAT11.3513513513513513No Hit
GAGCAAGAGCAGGTAGATATTGAAAGATGCGCCTTCTACCCGAGGTCGCA11.3513513513513513No Hit
GTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTCAACCCGATGA11.3513513513513513No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGATG50.0145.01
AATTATG50.0145.09
GATGTAA50.0145.04
AAGATGT50.0145.02
TAATTAT50.0145.08
AGATGTA50.0145.03
GAGAGAG50.0145.0145
ATGTAAT50.0145.05
GTAATTA50.0145.07
TGTAATT50.0145.06