FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7215116
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG78800.10921515329760463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA221450.082.593361
GCGAAAG97250.068.0802842
AGCGAAA100400.064.8919141
GAGCGAA60600.060.818971
AGCAAAA299850.059.9774252
GCAAAAG301800.059.5742033
CAAAAGC308150.058.059014
CGAAAGC118100.056.5987473
AAAAGCA359150.049.9144025
AAAGCAG478200.044.9007036
AAGCAGG446700.044.366097
AGCAGGG232300.034.1690988
ATACGGG16700.032.558963
TACGGGC17300.031.0078934
CGATTAC33350.028.2810481
AGCAGGC140300.028.1066978
GAAAGCA257550.026.626634
AGTAGAA96000.025.6198431
ACGGGCT21600.024.498915
GTAGAAA127500.022.5906281
Produced by FastQC (version 0.11.5)