FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10027617
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG110140.109836664084797No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA273050.077.0993041
GCGAAAG111150.071.8924262
AGCGAAA121550.065.590051
CGAAAGC137800.058.1832283
GCAAAAG380850.054.7998773
AGCAAAA388200.053.4595222
CAAAAGC391850.053.2216154
GAGCGAA73350.047.9663661
AAAAGCA469300.044.7004285
AAGCAGG551250.041.236777
AAAGCAG610200.039.6650436
AGCAGGG280350.031.7501958
AGCAGGC176800.026.9359118
GAAAGCA315050.026.5748334
AGTAGAA125850.022.3076041
AACAAGG219450.021.6429622
GTAGAAA166050.021.275771
GCTAGCC29850.019.9141333
AGCAGGT201600.019.7392398
TAGAAAC169750.019.6071262
Produced by FastQC (version 0.11.5)