FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9802733
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG434240.44297850405596073No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA309660.31589149678972184No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA277900.2834923689138529No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA214370.2186839119253784No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT209610.2138281232386927No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT206230.21038010522167644No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT194180.1980876149539113No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAACGGTGAAATTTCTCCAACT185170.1888963006541135No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG176730.18028645684830955No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC167160.1705238732912546No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC164260.16756551463760158No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG163730.16702484909055465No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAGAAAAGAATAGAGA155800.15893526835832414No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA154930.15804776076222823No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT154290.15739488161107723No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC153320.15640536164761398No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC138760.1415523609589285No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCGT128370.13095327598946133No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT127710.13027999436483684No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT125020.12753586168265524No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC122840.1253119920740471No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG119630.12203739508155531No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA117260.11961970197494924No Hit
GCAGAGGCCATGGAGGTTGCTAATCAGACTAGGCAGATGGTACATGCAAT114850.1171612039213962No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT111060.11329493519817381No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT110720.11294809314912485No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTTGAAAATTTGCAGG109290.11148931629577181No Hit
GTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATG109260.11145871258556161No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAAC107550.10971430110357999No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT106910.10906142195242897No Hit
GATTCGCTTGGAGAAACTGTGATGAGAATGGGAGACCTTCACTACCTCCA106090.10822492054001676No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105950.10808210322570246No Hit
GCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGAC104550.10665393008255962No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT104190.10628668556003718No Hit
AAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGT101190.10322631453901683No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTC101010.1030426922777556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA34500.0115.467211
GCAGGGT163200.072.9733059
GCAGGTA89950.072.6861959
CGAAAGC79750.068.265323
GCGAAAG79600.068.2313162
GTAGAAA113500.065.912571
GAGCAAA318600.064.294011
AAGCAGG393400.061.799077
AGGCTCG10550.059.13203145
AGCAGGG244200.059.0679368
AGTAGAA86850.055.811
TAGAAAC139550.053.4168472
GCAAAAG419550.048.760053
CAAAAGC437950.047.2711074
GTGTTTT119650.046.828849
AGCAAAA444350.046.002872
AGCAGGT189000.043.567338
AAAGCAG576800.042.665266
CAGGTAC27200.042.6424333
GACAGTA22800.040.057977
Produced by FastQC (version 0.11.5)