FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5618801
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTGTGTAGTTAAAACTAATACTCGGTGTCATCATATGT153150.272567047667287No Hit
ATATAATGGTACAAACGGGACTTAATTTCCGTAACGATGTGAATACCACA115700.20591581727133598No Hit
ATTCTAAAGTGAATAATTATAGCATACAATGGATACACACTCAAGATGCC112040.19940197205773974No Hit
CTCCAACAGACGCGGTTGAAGCTCTTCCAATCCTACACATCGATGAGCCA83840.1492133286087192No Hit
ATTTTACCTTGACTCTTTCAGCAAATACGAATAATGTACTGACTACCAAA81100.14433684339416897No Hit
ATCCTGAGATATGTCTATTTGGAGTAATCACTTCTCTATTGGTTGCAGCA78690.14004767209232005No Hit
CATTTCACACCATCGATTTCTTTTACGATAACTTCTGGACGAGATACTTG71010.12637927557854423No Hit
ATTATCAACCGATTGGCGAACACTTTGATTTAAAAATACTTACTGACATC69350.12342490862374375No Hit
TAATTATAGCATACAATGGATACACACTCAAGATGCCAAGGCAAATCCTT69340.12340711123244978No Hit
GTACATTATTCGTATTTGCTGAAAGAGTCAAGGTAAAATAGCGTAAAACA68800.12244605210257492No Hit
CATCTATACAAAGGGTAGTTGGGCTGTGAGGCCTGAGATTAATTATAGAA68210.12139600601623016No Hit
CCATTATATTTCTCAATAGCCCATCTTCTGCTCCGCCTTTTCGTTTAAAT67900.1208442868861168No Hit
TCTCTATTGGTTGCAGCATCATAACTTTTGGTGATAGTTTTGGTAGTCAG63560.11312021906452997No Hit
GTAAATATGCTGATTATTCAGCGAGTTGGTGTAGAAATTACTGCTATTGA62340.11094893732666454No Hit
GAGCAAAAGCAGGGATTCTTATTCCAAGTTTTGGGGAGAGAGAGACTGAC61750.10989889124031976No Hit
CTTTAGAATAGTCATCAAGTCCTTTTATCCCTCTTAAAGTGGAAGAAATT61650.10972091732737999No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG61440.10934717211020643No Hit
ATTCTATCTATTTAAACGAAAAGGCGGAGCAGAAGATGGGCTATTGAGAA61350.10918699558856063No Hit
TTTCTACACCAACTCGCTGAATAATCAGCATATTTACAATGGAAATAATC60920.10842170776291954No Hit
ATACAAAGGGTAGTTGGGCTGTGAGGCCTGAGATTAATTATAGAAAAAAC60600.1078521912415122No Hit
TCTCAATACTCAACAAAACTCGAGTATAAGTTTCACAAAGCGATTCCTCA56380.10034169211545309No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA152250.094.81931
GAGCGAA32150.092.738881
AGCAAAA219400.065.369922
CAAAAGC227800.063.070484
AAGCAGG268400.061.657797
GCAAAAG239500.060.0217743
AGCGAAA77950.056.862511
AAAAGCA254950.056.467745
AAAGCAG314600.054.9082536
CGAAAGC82000.053.573553
GCGAAAG83200.052.98692
AGCAGGT123150.052.9742668
GACGCGG34700.052.0147749
AGACGCG38500.047.4456758
AGCAGGG161800.042.0672348
GCAGGGT48650.039.03699
TAGAAAC57600.037.764792
TGATCGC5950.035.3311045
GAAAGCA126000.035.3243374
GCAGGGG55850.034.2639669
Produced by FastQC (version 0.11.5)