FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7816676
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCAAAGGTATGGTTCATGATACCGTAACCACGAGTCATTGACAAGAAC86340.11045615809072808No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG83350.10663100274336561No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA38100.093.503921
GCGAAAG102400.075.911862
AGCGAAA103850.074.8266451
CGAAAGC107700.072.156323
GAGCAAA221450.068.411351
CAAAAGC278650.053.485294
AGCAAAA288400.051.593722
GCAAAAG290550.051.0980573
AAAAGCA348750.042.9210745
AAGCAGG429600.040.7988937
AAAGCAG452300.040.7230266
GAAAGCA255750.031.5180554
AGCAGGG263550.027.1177488
TTTACGC34300.025.783154
TACGCCA34650.025.7314226
ACCGTCG19350.025.4721059
TTACGCC35250.025.2936045
TACGTTC57300.025.193974145
AGCAGGC156600.025.0869148
GCCATCG37800.022.8187649
Produced by FastQC (version 0.11.5)