FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCGAAAGCAGGTGTGTAGTTAAAACTAATACTCGGTGTCATCATATGT34.761904761904762No Hit
CTATTAGCACACCTGCTTCCGTTTGTCAAGCTGCTCCGTCCTCTAGTCAA23.1746031746031744No Hit
TCCCAAAAGTGGTATATCCTGAGATATGTCTATTTGGAGTAATCACTTCT23.1746031746031744No Hit
ATCTCCCACAGCCCTCTCAAACATGTGCTTTCTTCTCCAACCCCAGATCA11.5873015873015872No Hit
TAATGGTACAAACGGGACTTAATTTCCGTAACGATGTGAATACCACAAGT11.5873015873015872No Hit
CAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCTTTCAAGATC11.5873015873015872No Hit
TTCTTCCACTTTAAGAGGGATAAAAGGACTTGATGACTATTCTAAAGTGA11.5873015873015872No Hit
ATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGTTCCTG11.5873015873015872No Hit
GTATGCTGTTGGTAATCGCTTGGCTCGTTTGGCTCTTCAGAAGACCTATC11.5873015873015872No Hit
ATTAAGCCAAGAGATGATCAAAACGATGGCAGATACTCCGTTAATTCTTG11.5873015873015872No Hit
TAATGTGCCTCCAGGTCCATTGCTTGGATCATATTCACATAATGATTTTT11.5873015873015872No Hit
AGTAGAGACGGGAGACAAAATAACATTCGAAGCAACTGGAAATCTAGTGG11.5873015873015872No Hit
GTACTATGTTCTTTTCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGG11.5873015873015872No Hit
GTCAAAGATTGTAGATATTATTGGTAAATTTTTAACGCCTTTTCTTTTGG11.5873015873015872No Hit
CTTTTTGGAGTATCTCCGTACGGGTTACAAGCGCTTTATGGAAATCGGGC11.5873015873015872No Hit
TTGCATATGTCTACGGCTTGTTCCTCAGTTGGATTCTGTCTAAGGATGTC11.5873015873015872No Hit
GAAAAAGAGCGCGAAGAATTGCGTGAAATCATCACTGGAATTTTCCAACA11.5873015873015872No Hit
CATTGAGAGTGGGTATTCTTCTCCCAGTCCACTGCCTCAAATGTCAGTCT11.5873015873015872No Hit
GTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACCTTGAT11.5873015873015872No Hit
GTTTTGGGGAGAGAGAGACTGCCGGATTTTTCTTAAATGGCTTGGGATAT11.5873015873015872No Hit
ATTTTATCATCCGATTGGCGACCACTTTGATTTAAAAATTCTTACTGCCA11.5873015873015872No Hit
AGAGAGCACCTGCCTCCTGTAGGAAAGAAAGGGAGGTTATGCAACAGCAC11.5873015873015872No Hit
CTTTGAAAGGACTAAAGCCTGTTATCACTCGCCTGCTACAGCATGGCCTT11.5873015873015872No Hit
GGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGTACTATGT11.5873015873015872No Hit
TCGCTATACTACGCTCCTAGCTTTAAACGTAGAAACTAGAAATGAATCTT11.5873015873015872No Hit
AATATTAGCCTTCCGCAGGTGAATATTGCCGTTAATCAATTCTATCTATT11.5873015873015872No Hit
GAGCAAAAGCAGGGATTCTTATTCCAAGTTTTGGGGAGAGAGAGACTGAC11.5873015873015872No Hit
ATTCTCCACGTCCTGAAACAGTCCATTTATCTGGTGAATCAGTTGGGTCA11.5873015873015872No Hit
TCATAACTGCAAGTTAAACTCAGACCAAATGGGACTGGCTCAGTCAACCC11.5873015873015872No Hit
GTCTCGGGGGCCTTGTCTATAAAAAGGGGAAAATGCCTTCTAAGCTGGTG11.5873015873015872No Hit
TTGCCATGAGTCTTTTCTCGCCGGTATTGGGAACCTTCCTTATCTTGCGT11.5873015873015872No Hit
CTTCCATTTTCGGAGAAACGCTCAGCAGGGATTCTTATTCCAAGTTTTGG11.5873015873015872No Hit
CGTTTAAATAGATAGAATTGATTAACGGCAATATTCACCTGCGGAAGGCT11.5873015873015872No Hit
TCTACACACTCTGCACATGTATCCTGGAACTTAAAGTAAAATAAAAATAA11.5873015873015872No Hit
GTACAGAGGTCCTGACAGAGAGCCATGAAATGTCTTTAGCCATGAAATGT11.5873015873015872No Hit
CTTCTACCCAATTCAATTCCATCCATCCATCCATCCATGTTAAGTGAGCA11.5873015873015872No Hit
ATTCTAAAGTGAATAATTATAGCATACAATGGATGCACACTCAAGATGCC11.5873015873015872No Hit
ATCTCCCACAGCCCTCTCAAACATGTGCTTTCTTCTCCACCCCCAGATCA11.5873015873015872No Hit
ATACAATGGATACACACTCAAGATGCCAAGGCAAATCCTTTCTTTAACTT11.5873015873015872No Hit
GTAGCAATTACTGCGAGTGCCGATATTCCCTGAGGCCGAAAAGTTACAGA11.5873015873015872No Hit
GTTCCTAACAGGCCACAGCCCGGTACCGGCCCATGGCCCAGAGGTCGTGG11.5873015873015872No Hit
CATCACAGTTTCTCCAAGCGAATCTCTGTATATTTTCAGAGACTCGAACC11.5873015873015872No Hit
GAGCTGTATAGTGTGTTTACAGTCTAGGTTCAGAGTTAACTTAAAAATAA11.5873015873015872No Hit
CAATTAAATCAAATACGAGATTAAATAGTTCAATACTTTCACAAGATTCT11.5873015873015872No Hit
GGTGTGAGGACAAGTGTCTCAAACGTCAACTGCACATATAAGAAGTAGGT11.5873015873015872No Hit
CCTTTCTGACATGACCCTCGAGGAACTGTCACGAGACTGGTTCATGCTCA11.5873015873015872No Hit
CATTTCACACCATCGATTTCTTTTACAATAACCTCTGGACGAGATACTTG11.5873015873015872No Hit
CCTTGGTGCTGCAGTGTGAGAGCTGGGTCCCATTGCTGCAGACACTTCTT11.5873015873015872No Hit
CTTTCGCTCCCCCCGGAGCAAAAGCAGGTGTGTAGTTAAAACTAATACTC11.5873015873015872No Hit
CTCCCCAAAACTTGGAATAAGAATCCCTGCTGAGCGTTTCTCCGAAAATG11.5873015873015872No Hit
TTCGTGATGAAATCATTAAAAAAGGAGGCTGGGTAAATGCACACGCTCAC11.5873015873015872No Hit
GATATTTATGGGAATTTGGACTCCCGTTTTCATCTCGTCCCACATTTCTT11.5873015873015872No Hit
ACCAGTAACAATTAGTTCCGTGGCTCTTGGCCAAGCAGTGCTAATGCCTA11.5873015873015872No Hit
TTCTAAGTGTCTCGCTGGATTCCTCTTTCAAGATCCATTCCACGATTTGT11.5873015873015872No Hit
GACGACCACCAATTTCCCCTGGAATCAATGGCAAGTATTGGTCGAAGGCA11.5873015873015872No Hit
GGATATTATCAACCGATTGGCGAACACTTTGATTTAAAAATACTTACTGA11.5873015873015872No Hit
TCGAAACAATGCGTCGTGAGGGATATGAACTTCAAGTATCTCGTCCAGAG11.5873015873015872No Hit
GGCAAATCCTTTCTTTAACTTTTCGGCCTCAGTGAATATCGTCAATAGCA11.5873015873015872No Hit
CCATAAATATCTACAATTTGTATGTTAATTAAATAAATAAATACCAAAAA11.5873015873015872No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACCT50.0145.08
ATTAGCA50.0145.03
ACACCTG50.0145.09
TAGCACA50.0145.05
GCTGCTT50.0145.0145
AGCACAC50.0145.06
TATTAGC50.0145.02
GCACACC50.0145.07
TTAGCAC50.0145.04
CTATTAG50.0145.01
Produced by FastQC (version 0.11.5)