FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5394292
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA190710.35354037193388865No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG163220.302579096570968No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC141110.2615913265355305No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT140290.26007120118821897No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC132880.24633445872044007No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA105950.19641131774104925No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC105500.19557710261142702No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT99520.18449131044444758No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT97020.17985678194654645No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA91240.16914175205939908No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC86880.16105913435905955No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA86360.1600951524314961No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC82510.1529579785447284No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT81120.15038118069989537No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA77810.1442450649686743No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC77610.1438743026888422No Hit
GTATAGGAAACTTAAGAGAGAGATAACGTTCCATGGGGCCAAAGAAATAG74020.1372191197658562No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT73400.13606975669837673No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG72990.13530969402472096No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG70920.1314723044284588No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA67980.12602209891492713No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT64830.12018259300757171No Hit
GTCATACTCTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA64370.1193298397639579No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG63200.11716088042694017No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG62770.11636374152530118No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG62750.11632666529731799No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG62610.11606713170143551No Hit
TCCTAGTTCCAGTACTGGTTTAAGAGATGATCTTCTTGAAAATTTGCAGA60680.11248927570105585No Hit
GTATACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCAGGCAT59010.10939341066445792No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG57080.10581555466407824No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA56460.10466619159659876No Hit
TCCTTACTCTGGGTCTAGATCCGATATTCGGGATTGCAGTTTGTTGGCTT56210.10420273874680866No Hit
GGTTTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT56080.1039617432649178No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA55950.10372074778302695No Hit
CTCCTGTTCCACTTCAAACAGCAGTTGTAATGCTTGCATGAATGTTATTT54770.10153325033201763No Hit
GGTGTGGAGACCATGGTCATTTTAAGTGCCTCATCAGATTCTTCTTTCAG54490.10101418314025269No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCTCG3900.083.69342145
GCAAAAG129200.083.1540453
GCAGGGT59850.080.180259
GCAGGGG22200.076.0810559
AGTAGAA24200.075.847491
GAGCGAA28800.074.0612261
CGAGTCG2050.063.68865145
GCGAAAG36950.063.3908732
AGCAGGG102700.063.2428058
CGAAAGC37350.062.4975473
CAAAAGC181400.060.7818034
GAGCAAA196200.056.057821
TCACGCG553.2127628E-452.752213145
AGGGTGT36100.052.6113856
AAGCAGG245700.052.57517
AGCGAAA45550.051.2865521
AGCAAAA215900.050.550162
AGCAGGC8150.048.919128
TAAACTA10200.044.062915
CGCCGTT1652.7284841E-1143.96018145
Produced by FastQC (version 0.11.5)