FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8471308
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG87400.081.728942
AGCGAAA88250.080.24721
CGAAAGC92250.077.876623
GAGCAAA181750.059.3651161
GAGCGAA38900.053.9067341
GCAAAAG254300.041.9909973
CAAAAGC258150.041.6431124
AGCAAAA287200.037.19372
AAGCAGG387500.031.314847
AAAAGCA355100.030.9266995
AAAGCAG425500.030.0688466
GAAAGCA252200.029.863774
AGCAGGG250550.023.5792337
AGCAGGC133000.020.3832598
TGACGCG8300.018.352375145
GCCGACC18850.017.316187145
TATACTG64600.015.1486115
GTATATA93650.015.1085091
TCGCTCC18000.014.9004995
GCAGGGG147300.014.8613368
Produced by FastQC (version 0.11.5)