FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8257364
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT177730.2152381801262485No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT176440.21367593822919762No Hit
GTACAAATTGCTTCAAATGAGAACATGGATAATATGGGATCGAGCACTCT163420.19790819443105573No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC151140.18303662040331514No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC142840.17298498649205726No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA119860.14515528200040595No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA118070.142987519988219No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT115980.14045644590695044No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG115520.1398993674010253No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC100370.121552107912404No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA98030.11871827377356746No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG95390.11552112756564928No Hit
GGAGTGGAGGAAACACTAATCAACAGAGGGCCTCCGCAGGCCAAACCAGT95150.11523047791038399No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAGAAAAGACG92790.112372422966942No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA90610.10973235526494897No Hit
TGATAACACAGTTCGAGTCTCTAAAAATCTACAGAGATTCGCTTGGAGAA88690.10740715802282665No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG88580.10727394359749673No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT88320.106959073137626No Hit
TTCTTAATCTGTGTCTCACTTCTTCAATTAGCCATCTTATCTCTTCAAAC86460.10470653830932002No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT85210.10319273802147998No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT83800.10148517129679642No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA75350.080.980061
CGAAAGC82350.074.387163
GAGCGAA33650.067.272091
GCGAAAG93200.065.745192
GCAAAAG207100.054.8171583
GAGCAAA237800.049.3664631
CAAAAGC248400.046.2847634
AGCAAAA273650.042.1067472
TAGAAAC111750.041.724072
AGTAGAA81450.038.0366361
GCAGGGT91100.036.1243679
AAGCAGG369850.035.7492757
AAAAGCA325300.035.476245
AGGGTGT82150.034.0608636
CAAGGGT102550.033.9306374
CAGTACG12450.033.1870239
TCGTACT42650.032.3041082
CGTACTC43150.032.0890923
ACAAGGG112200.031.9828533
AGCAGGG209550.031.4442798
Produced by FastQC (version 0.11.5)