FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8670085
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA129030.1488220703718591No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA119580.13792252325092544No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT112600.12987185246742103No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA97610.11258251793379188No Hit
GTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATG89950.10374754111407213No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG104400.076.337912
CGAAAGC111150.071.810123
AGCGAAA113600.069.878781
GAGCGAA36650.068.7008061
GAGCAAA200750.062.1336141
AGCAAAA297650.041.685712
TAGAAAC116850.040.2771872
CAAAAGC314800.039.5841564
GCAAAAG317700.039.1796843
GTAGAAA131250.036.76451
GAAAGCA232550.035.1931274
AAGCAGG411500.034.1206677
AAAAGCA409450.030.645485
AGTAGAA120000.029.387411
AAAGCAG512250.029.1228356
AGCAGGG214250.027.979667
CTACACT63450.025.4773064
GGGTGTT104550.024.8901867
CAGTAGG80400.024.7024339
ACAAGGG154950.024.5158823
Produced by FastQC (version 0.11.5)