FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGAATACATAATGAAGGGAGTGTACATAAATACGGCCTTGCTCAATGC23.7037037037037033No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAAC11.8518518518518516No Hit
CAGAAAGGTAGCGCGAAGTAGGTACAGATGCAATTGTCATTCTGAGTGTC11.8518518518518516No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA11.8518518518518516No Hit
GTGAAAGGAGTTGGAACAATAGCAATGGAGTTAATCAGAATGATCAAACG11.8518518518518516No Hit
GTTCAGGCACTTAGGGACAACCTGGAACCTGGAACCTTCGATCTTGGGGG11.8518518518518516No Hit
GGCGTGGATTGTCTCCGAAAATCCCACTGCATATGTATCCTATCTGATAT11.8518518518518516No Hit
TGGCCGGATCGAGTGAACAGGCAGCAGAGGCCATGGAGGATGCGAATCAG11.8518518518518516No Hit
CCACTAATCAGGCATGAAAACAGAATGGTGCTGGCTAGCACTACGGCAAA11.8518518518518516No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT11.8518518518518516No Hit
CAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAATTTCT11.8518518518518516No Hit
AAATCGGAATGGGGACCCGAACAACAAGGAAAGAGCAGTTAAACTATACA11.8518518518518516No Hit
GCAATATCCTCAAAGGAAAATTTCAAACAGCTGCCCAGAGGGCAATGATG11.8518518518518516No Hit
GATCAGTGTGCAGCCTACAATCTCAGTGCAGCGAAACCTCCCTTTTGAAA11.8518518518518516No Hit
TCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGCAATTTCTCCAACTAT11.8518518518518516No Hit
TAGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCAAGCGAATCTCTGTA11.8518518518518516No Hit
CTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTT11.8518518518518516No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG11.8518518518518516No Hit
TAATAAAACTTCTCCCCTTTGCTGCTGCTCCACCAGAACAGAGTAGGATG11.8518518518518516No Hit
GTGTTAAAGTACAACGGCATAATAACAGACACTATCAAGAGTTGGAGAAA11.8518518518518516No Hit
GGCCAAGGGTGTTGCCTCTTCCTTTTAAGGAATTTTGATCTCGGCGGAGC11.8518518518518516No Hit
CACAGCACTCTGCTGTTCCTGTTGATATTCTTCCCTAATGGACTCAGGCA11.8518518518518516No Hit
CTGCCCATTTTGATGGTGATAACCGTACCATCAATCTAACATCCCTGTCC11.8518518518518516No Hit
TTTCAAATGATGACACTGAGCTCAATTGCTCTCTTAGCTCCTCATAATCG11.8518518518518516No Hit
GAGCAAAAGCAGGAACTAGTGATCCGGCCATGGCACGTGGGTGCGTGGTC11.8518518518518516No Hit
AAACAGAATGGTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAGATGG11.8518518518518516No Hit
AGCATGTACGCCACCATCAAGGGAGCAATTTTACAATCCTGGAGCTCTTC11.8518518518518516No Hit
GTCCTGTAGCTCTGCTAGCACCTGCTTCCAAGCCATGAGGTAATTGGGAT11.8518518518518516No Hit
GTCCAGTATCGTCTAATGGAGCAAATGGAGTAAAAGGATTTTCATTCAAA11.8518518518518516No Hit
ATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACAACCATA11.8518518518518516No Hit
ATTTCTTTCACAATCACTGGGGACAACACTAAGTGGAATGAAAATCAAAA11.8518518518518516No Hit
AGTAGAAAAAAGGGTGTTTTTTATCATTAAATAAGCTGAAAAGAGAAACC11.8518518518518516No Hit
AGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGAAAGGTA11.8518518518518516No Hit
GTGCAGCGAAACCTCCCTTTTGAAAGAGCAACCGTTATGGCAGCATTCAG11.8518518518518516No Hit
AGCAAATGCGGGATGTGCTTGGGACATTTGACACTGTCCAAATAATAAAA11.8518518518518516No Hit
TATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTC11.8518518518518516No Hit
CCATCCATCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATG11.8518518518518516No Hit
TTGCATCTGTACCTACTTCGCGCTACCTTTCTGACATGACCCTCGAGGAA11.8518518518518516No Hit
GGGTAAAGCTGGAATCAACAAGGATTTACCAGATTTTGGCGATCTATTCA11.8518518518518516No Hit
CTCGAAGACTCCCCGCCCCTGGAAGGACAAATCTTCTGGCTTTGCACTTT11.8518518518518516No Hit
GTGCCTCCTTGGCACTGAGATCTGCATGGCCAGGGTTTGTATCAACTCTC11.8518518518518516No Hit
CGTCAATGGCATTCTGTGTGCTCTTCAGGTCGGCTGCATATCCTGACCCC11.8518518518518516No Hit
GATCTTGAAAGAGGAATCCAGCGAGACACTCAGAATGACAATTGCATCTG11.8518518518518516No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTC11.8518518518518516No Hit
GCTCAGTAGGGTTCGATATGGGCTCCTGTCTTTAATGGTTCCAGTGGAAT11.8518518518518516No Hit
ATATATTGTTTCTCCAACTCTTGATAGTGTCTGTTATTATGCCGTTGTAC11.8518518518518516No Hit
ATCTGGACCCCTCTTGTGGAAAGCTTTCCTCTTGGAATCACTTTCTTTCC11.8518518518518516No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT11.8518518518518516No Hit
CATCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTTGAAAATTTGCA11.8518518518518516No Hit
GATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGTGTGTGCCACTT11.8518518518518516No Hit
GATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTT11.8518518518518516No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTCG11.8518518518518516No Hit
GTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGAGAATAGAACGATTC11.8518518518518516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTCTG50.0145.08
CCTGTAG50.0145.03
TAGCTCT50.0145.07
GTCCTGT50.0145.01
TGTAGCT50.0145.05
GCTCTGC50.0145.09
TCCTGTA50.0145.02
CTCATAT50.0145.0145
GTAGCTC50.0145.06
CTGTAGC50.0145.04
Produced by FastQC (version 0.11.5)