FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATGGGCTCATGGACCATTCAGTGAACGGCTGGGGGGTAGGAAAGAGGC11.9607843137254901No Hit
CGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCACAATATCAACTG11.9607843137254901No Hit
TTTGTTGTAAGTGTTCAGGCAGATCTTGAATCCTGGGAATGATGCGCTGG11.9607843137254901No Hit
GAAAAATACAGAGATAAATAATTTATACACCGTTTTCATTTCAATTAAAT11.9607843137254901No Hit
CTTCCCATTAGGACACACCTCCAACATTGGGTATCGAATTTCAACAAAGG11.9607843137254901No Hit
GAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATGGTAAATGGCAACT11.9607843137254901No Hit
AGGTTGGCTTGAGCCCAGGAGTTTGAGACAAGCCTGGGCAATATGGCAAA11.9607843137254901No Hit
CTATAAAGAGAGGATAAAGCTACATATCTACAACTGAAATAAATACTATA11.9607843137254901No Hit
ACTAAAGCTTGAAATGGATCCACTTCAATGATGTGGTCCTACTTTCCAGG11.9607843137254901No Hit
CCTCTGCATCCAGTTGCTCTGTATTTTAAGCATTTTATGTATAGTACTAT11.9607843137254901No Hit
GGAATGATTGGATGCATGTGATTTGTTCTCAGCTATTTTCAGTTGGTGTT11.9607843137254901No Hit
ATTGGGAGCCGATGATCGCACTTGGGTCGACTAACTTCGGGAAGGTCGCC11.9607843137254901No Hit
ACACATACCTACCAGGAGGAGAACCTGCGGAATTTCTCACTAAAGTATTA11.9607843137254901No Hit
AAGGAAGAGAGGTCCAACAAAAAGCTGGAAGCAGAAAAATTGGGTCTCAT11.9607843137254901No Hit
GCATCATTAGCTATTCTACCTGATGCTTGCCCTCCCGCCATTCCTCTGAC11.9607843137254901No Hit
ACGTATAACAGGTCTGGTTCCTCTCTTAGGGTTACTTTCATTTCTTTAGG11.9607843137254901No Hit
GTATTTATCGTCGCTTTAAATACGGTTTGAAAAGAGGGCCTTCTACGGAA11.9607843137254901No Hit
CTTTCATGTCTTTTACGTATGTTTTTTCAAATTCCTTGCCGCGATCTCTA11.9607843137254901No Hit
GTGTTAGATCATGGTCCAAGGAACTCCGCAAGCCACATCAGTAAGAATAC11.9607843137254901No Hit
GGTATGTGCAACATGTGAGAAGATTCCTTACTCCCAGCACAGGTCTCATA11.9607843137254901No Hit
CGGCCTCCTTTGCTGGGCTTTGTTTTTTGTCTTTTTCGCCCCTTCTCGCT11.9607843137254901No Hit
GATCAATTGTATGTTGGTTCTCCAGAGCAACAAGAAGCTCCGCGTTGTAT11.9607843137254901No Hit
GCTCCAGAGAATATTAGGACATTTGTTTTTTTCTCCTACTATGTTTGCTA11.9607843137254901No Hit
CATCAGCAACACAAACTTTGCTGCTGGACAGTCAGTCGTTTCCGTGAAAT11.9607843137254901No Hit
CGTGTTTTTTATCATTAAAGAAGCTGAAACGAGCAAGCTCTTATCTCTTG11.9607843137254901No Hit
ATATTAACCTTAAATGTAAATGGACTAAATGCTTCAATTAAAAGACACAG11.9607843137254901No Hit
CTGTATATAGTCAGATCCTCTAAACGTCTTACAATTCAATTCAAGTCCTC11.9607843137254901No Hit
ATGGGAGACATTCACTACCTCCAGAGCAGAAATGAAAATTGGCGAGAGCA11.9607843137254901No Hit
TTCTAGAAGCGCAAATTGCAGAAGGCAGGGCCTCTTTCCCTCCGAAGGCT11.9607843137254901No Hit
AGCGAAAGAATTGTGACAAAGACAGAATGTATTCAAAAACTTTTTCAAGT11.9607843137254901No Hit
ACCTATGATTACATCACTGAATACCACCCTGCACAACAGCAGTAAAACCT11.9607843137254901No Hit
GCAGTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTT11.9607843137254901No Hit
ATGGCCTCGCGGCGCGTTTCTATGAGACCCGCGATTGCGGCAAATATGGG11.9607843137254901No Hit
CAAAAAAACAAAATAAAATGAGGCTTAACACCAGCTCTATCAACTTCATT11.9607843137254901No Hit
GAATAGAACAGTTAATGGGCCATGCATCAAAGCTTTATATTAGTTTATCT11.9607843137254901No Hit
CCCTTCTTTTTAATTTGCCTCTCTCTGCATCTTTGGTCATCGTGTTCAAT11.9607843137254901No Hit
CTATTGAGGAATTGTCAAGAAACTGGTTCATGCTAATGCCCAAGCAGAAA11.9607843137254901No Hit
GGACGCTGCCGACGATCTACTCTGTGTAGATCTCGGTGGTCGCCGTATCG11.9607843137254901Illumina RNA PCR Primer (96% over 27bp)
TTACAATACCATTCACGTACACCGATCAGGACCCCAACTGCATTTTTGAC11.9607843137254901No Hit
ACTCTATGAGCAGTTAGTTGAGTTGATAAATACAGGATAGACAAAAGAGT11.9607843137254901No Hit
CTGATGGAGTAGCAGGGCTGGGGGGGGTAGGTCTAGGGCCGGGGGGGCCT11.9607843137254901No Hit
GACTAAAATTGGGGGCTCAGAAACGCGGAGCGAGAAACTCCAACAATGAA11.9607843137254901No Hit
ATTCAAGTCATCAGATGAAGACGCAAAATGCATATTATACATCCACAAGA11.9607843137254901No Hit
TCTTTAAATAAGGTTTGAAAAGATGGCAGGAGACGTAAGGAGTTACTGAG11.9607843137254901No Hit
AAAGCAGGGAGCTGGCAAAGCAGATGGGAGTCCTTCCAACCCGGAACATA11.9607843137254901No Hit
GCAAAAGCAGGATATAGCGGGAGTTTTGTTCAGCATCCAGAACTAACAGG11.9607843137254901No Hit
GTTCATCGAAAAGTGAAATATGCGACAATCCTCATCAGATACTTGATGGA11.9607843137254901No Hit
ATATGGGCCAGCCCTCCTCTGGGTGACATGACACACACATGAACAAGACC11.9607843137254901No Hit
GGTTGTTCCTGGTTGGCGGTGCTTGCAGTGAGCGGCGATCACGCCTCAGA11.9607843137254901No Hit
TCTGTCAAAATTATGAATTTGCTACCAATGATTCATACTTGTTTAAACAT11.9607843137254901No Hit
AGCAGATAGTGGTAGCATGATTAGATGTTTCAAAATCTTTTTCCTTGATG11.9607843137254901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTGGC50.0145.02
TAGCTAC50.0145.0145
TTGAGCC50.0145.09
GGCTTGA50.0145.06
GCTTGAG50.0145.07
AGGTTGG50.0145.01
TTGGCTT50.0145.04
GTTGGCT50.0145.03
CTTGAGC50.0145.08
TGGCTTG50.0145.05
Produced by FastQC (version 0.11.5)