FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2075612
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATAACACTGAAATTCGCTT29530.1422712915516002No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC29410.1416931488158673No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG28460.13711618549131532No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC26210.12627600919632379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA12700.090.254531
GAGCAAA67700.075.546811
AGCGAAA22250.065.536261
GCAAAAG77450.064.862333
AGCAAAA78900.063.2307322
CAAAAGC80950.062.0549134
CGAAAGC24450.059.8898473
GCGAAAG25700.057.8412252
AAAAGCA103850.048.578225
CGAGTCG604.931335E-448.36308145
AAGCAGG128350.045.2915657
AAAGCAG140300.042.880446
AGCAGGG66700.039.8812378
AGTAGAA31250.039.2330741
TAGAAAC37600.037.2208442
CTTAGCG4150.036.6933982
CATACCG2252.0008883E-1135.43559
GTGCGTC1751.0121221E-733.1352926
CGCGGAT1102.4406613E-432.9462288
GTATCGT900.003617778432.242831
Produced by FastQC (version 0.11.5)