FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATCATATCAGCCTACTCAGAGCCACAAACAGCAAAATCTCCCCGACAT27.142857142857142No Hit
CACCACAGAGCCCAGGCATGCTGAGTCAGGCTGTAGACTGTTAATGCTAG13.571428571428571No Hit
ATAGGATGTGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTGCA13.571428571428571No Hit
CTACAGTCACGTTTTCTTTTCATTACAAACACAACGTCTCCATGCCCGAT13.571428571428571No Hit
GTATACATCATGGTCATAAGTTCCATTTCTGATTGATCCTATGCAGGCAT13.571428571428571No Hit
CCCCTCCCTCTTCTTCATTCTTCCTAATTTATTAACCTCCTAAACAACAG13.571428571428571No Hit
TCTTATTTCTTCAAACTTTTGACCTAGCTGTTCTCGCCATTTCCCGTTTC13.571428571428571No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT13.571428571428571No Hit
ATTTGTCAAATTGTTCATTGTTTGGCATAGTCACGTTCAATGCTGGGTAT13.571428571428571No Hit
TAGGTGTTGTCGCCATGTGCCGTTTCTGGTTTGGGGTGAGGGGAGCTCCG13.571428571428571No Hit
CTCTTGTCTGACCCCTCGAGCTAGGACTTTCGTACAATCTTGAATAACAG13.571428571428571No Hit
GGTCACTTGAATCGTTGCATCTGCACCCCCATTCGTTTCTGATAGGTCTG13.571428571428571No Hit
AGAAACTGGTTCATGCTAATGCCCAAGCAGAAAATGGAAGGACCTCTTTG13.571428571428571No Hit
CACCATTGTTGGCTTGCCGCCAGATTCGCCTTATTTCTTCTTTGTCATAA13.571428571428571No Hit
CTCTTATTCACTCTTTGTTTTTTCTTCCCTATTGTTCTTTGTGTGACCAT13.571428571428571No Hit
GATTGGATTCAGGGCTCCGCATGGCATGTACATATGTACCTCTGTGTGTG13.571428571428571No Hit
GTTATTAACTTTGTAGCAACTGTGAATGGGAGTTCATTCATGATTTGGCT13.571428571428571No Hit
GCAGTGAAGGAGAGCATGGGCATAGGCGAAGATTTGGGTAACAGCAATGG13.571428571428571No Hit
AGCTGAGAGAATTTAAGTAGTGTTGAATGTCAAAGAATTACTGCATACTT13.571428571428571No Hit
TACCAAGAGACTGAGAAACTGCTTGGGTGGTTTGAATAATAAAGGCTAGT13.571428571428571No Hit
GGACCAATACATGGGTACCACACTGGGTGCTGAGACTTGGCCTTCTTCAC13.571428571428571No Hit
TGGAAGCACACCCCAGCGCGGGGAAAGATCCCAAGAAAACTGGGGGGCCC13.571428571428571No Hit
GAATGGACCAGGCAATCATGGAGAAAAACATCATGTTGAAAGCGAATTTC13.571428571428571No Hit
GGTCACTTGAATAGTTGCATCTGCACCCCCATTCGTTTGTGATAGGTCTG13.571428571428571No Hit
GAAACAAGGAGACCACTAGAAGGCAACTATAGGAGTCTATGTGAGAAATT13.571428571428571No Hit
GAGGAGAAGAGGAAGAGGGAGGAGGAGGAGTGGAGGAAGGGGGGAGGAGG13.571428571428571No Hit
GGAAGTGGGAATGGCTCTAAGCAGCCACACAGAGCAGGAAATGAACTCAC13.571428571428571No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)