FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4292913
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG50150.080.250232
CGAAAGC50650.079.5798653
GAGCGAA20850.073.428361
AGCGAAA54350.073.292621
GAGCAAA102100.064.243641
CAAAAGC144350.045.4484834
AGCAAAA150100.043.189782
GCAAAAG156100.041.8436623
AAAAGCA184250.035.605625
AAGCAGG210550.035.5645647
GAAAGCA115500.035.3357244
AAAGCAG237600.033.499126
AGCAGGG138000.026.053738
TATACCG2301.1058764E-625.2138775
TACGACG1500.001496388924.1630177
GCTTTCG12400.023.9910681
ACAAGGG56600.023.695353
AGGGTGT53050.022.1364046
GGGTGTT48150.021.8295177
CTTTCGC14000.021.2363572
Produced by FastQC (version 0.11.5)