FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7685845
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC92200.090.030623
AGCGAAA95250.086.904371
GCGAAAG110200.075.534722
GAAAGCA209000.040.65134
AAAGCAG315150.035.3999865
AAGCAGG289450.034.8616146
AGGTGCG28100.031.7299427
GGTGCGT27800.031.550858
GTGCGTT29000.029.495419
AGCAGGG194750.028.0649577
GAGCGAA30150.027.6714231
TACGTGC11950.023.657417
CGCCGTT6950.022.960264145
GCAGGGC63700.021.7352398
TAAGGTG62950.020.1522755
TGACGCG11900.019.504822145
GAGCAAA147900.019.0320031
GCAAAAG157700.017.5149543
CGTAGAC21350.017.3176123
AGCAGGC94750.016.5251357
Produced by FastQC (version 0.11.5)