FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4408075
Sequences flagged as poor quality0
Sequence length151
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA111790.2536027631108817No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG100310.22755964905315815No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT86580.19641226612523605No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC85060.19296404893292424No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC71050.16118146810115527No Hit
GAGCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCT57870.13128179534150394No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAA56970.12924008779342458No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA56470.12810580582226935No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC55430.1257464993222665No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA52480.11905423569245079No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC49390.11204437311071158No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC48120.10916329690397737No Hit
GCGTGAATACAAACCCCAAAATCCCCTTAGTCAGAGGTGACAGAATTGGT46700.10594193610589657No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA46240.10489839669243378No Hit
GGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCT44370.10065618212031328No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA29450.089.6637951
GCAAAAG156800.079.0162053
GAGCAAA188200.067.224551
CAAAAGC190950.065.1460654
AGCAAAA202400.061.4401972
AGCGAAA48200.059.1488841
AGGCTCG5850.055.79225145
CGAAAGC52500.055.235823
GCGAAAG52450.054.7455332
AGTAGAA52850.054.6309971
AAGCAGG283600.051.7861867
AAAAGCA252100.049.6023145
AGCAGGG136100.046.3374378
AAAGCAG321400.046.3276446
CAGTACG22700.044.7067459
TAGAAAC82100.043.7180752
GCAGGTA81500.043.048569
GCAGGGT72250.042.0384839
CGAGTCG1859.640644E-1139.205368145
CAAGGGT42050.038.6164744
Produced by FastQC (version 0.11.5)