FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4890908
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG258490.5285112702999116No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA156560.3201041606180284No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA136040.27814876092537416No Hit
CATCACAGTTTCTCCAAGCGAATCTCTGTATATTTTCAGAGACTCGAACC124510.2545744062247746No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA91410.18689781120397275No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT89890.18379000381933172No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC89600.18319706688410414No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT88630.181213795066274No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC83250.17021379261274186No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC82350.1683736435034149No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT78880.16127884638189882No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA74420.1521598852401231No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG73640.15056508934537308No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT73110.14948144598099167No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA70910.14498330371374804No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT66010.1349647141185236No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT65190.1332881338189146No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC65170.13324724161648513No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT63850.13054835625613895No Hit
ACTATGATGAGAATGGGAGACCTTCACTACCTCCAGAGCAGAAATGAAAA58750.12012084463661962No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC58510.11963013820746578No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG57520.11760597418720613No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT55120.11269890989566764No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG52650.10764872289562592No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG51160.10460225381462909No Hit
GGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGA50540.103334595539315No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG50070.10237362878222205No Hit
GAGCAAAAGCAGGGGTCAGGATATGCAGCCGACCTGAAGAGCACACAGAA49100.10039035696439189No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA28200.0108.841451
GAGCAAA155850.086.924181
GCAGGTA48050.069.0939259
GCGAAAG60550.067.190432
CGAAAGC62700.065.095813
AGCAAAA220550.060.633492
GCAGGGT111600.060.2120329
AAGCAGG270500.059.7075167
CAAAAGC244650.054.786964
AGCAGGG182150.053.883688
AGCGAAA74200.053.7850881
AGGCTCG7500.053.19069145
GCAAAAG257400.052.1608433
GTAGAAA68550.051.5495151
AGTAGAA48900.049.26431
TAGAAAC72900.048.147812
AAAAGCA287300.046.804695
AGCAGGT94000.044.7268078
AAAGCAG376550.043.6425326
ACAAGGG91450.041.5393523
Produced by FastQC (version 0.11.5)