FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6316894
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA37450.072.657311
GAGCAAA182500.070.970081
AGCGAAA68300.064.486371
CGAAAGC70600.062.841163
GCGAAAG75200.058.918012
GCAAAAG225050.056.7254373
AGCAAAA226550.056.1423072
CAAAAGC231650.055.2000774
AAAAGCA278700.046.2455
AAGCAGG323050.046.2008257
AAAGCAG359750.042.8174936
GCTTTCG23200.038.7823031
AGCAGGG191000.035.5982938
TCGCTCC24050.035.2647445
CTTTCGC29650.029.837322
CGCTCCC28700.029.2980826
TCCCGTC47050.027.8961122
CTCCCGT53050.024.756721
AGCAGGA138250.024.4332078
CCGTCTG52150.024.186244
Produced by FastQC (version 0.11.5)