FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAATAAAAGAAGGAAGGTAAGTATAAGAGAAAACAGAAAATGTATTCAG23.8461538461538463No Hit
GTCCGTACCCGGGTGGTGAACCCCCCAAATGTACAATTTGTCAAATTGTT11.9230769230769231No Hit
AAACAAGGGTATTTTTTCATTAATTGTCATACTCTTCTGCATTGTCTCCG11.9230769230769231No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA11.9230769230769231No Hit
GGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGTTTCCAGGTAG11.9230769230769231No Hit
GAAGAGGACAAGCAGCAGATCTCAAAAGCACTCAAGCAGCAATCGATCAA11.9230769230769231No Hit
GATTAGAAGATACCTCTACTCCATCCTTGGCGACTGATCATGCAACCCTT11.9230769230769231No Hit
AATCTGTGATGTGTATTTAACATGAAAACGGAGATGCCAAATAGGCACCG11.9230769230769231No Hit
AGCGTGAGTCAGTTACAGGCACCCAGGTATCACCTACAAACTCAGCAGTT11.9230769230769231No Hit
GGCATGGGATGAGCAGAGAAATGCAGGGAGAGGCATTTGGGGAAACAGGA11.9230769230769231No Hit
GATGATTGATGGAATTGGGAGATTCTACATCCAAATGTGCACTGAACTTA11.9230769230769231No Hit
ATAATACATTCCCACGTTGGTCTCGAACTCTCAAAAACCGATCAATGCTA11.9230769230769231No Hit
AAACACACACTTAATCAGAAGCAGGGATAGGTTGGAAAATAGATCTGAGG11.9230769230769231No Hit
AGTTTGAAAGGGTCTATTCCCACCAAGGAATATCCCTCTTTTTCGAAGTC11.9230769230769231No Hit
GTCCAGAATTTGGCATCAAGGATGGAATTGCAGACAAAACTTTGAGAACC11.9230769230769231No Hit
GATAACAGATCCTTGGTATTCTTCTGGAGTGTCGATTTGTACACGTTCAA11.9230769230769231No Hit
GGATCTGAAAATTGAAACAAACAAATTTGCAGCAATAAGTCAGTATTCAA11.9230769230769231No Hit
AACAAGAAGTGCTTATGAGAGAATGTGCAACATTCTTAAAGGAAAATTTC11.9230769230769231No Hit
TCTCGCACTCGCGAGATACTGACAAAAACCACAGTGGACCATATGGCCAT11.9230769230769231No Hit
GCAGAGAACCATTAAAATATATTTAATACCTTTAATGTCAGTACCAACAA11.9230769230769231No Hit
GGAGTTACGTGCCCACGGTCCCGGAGCAAGGATGAGAACTGGGAATAGAC11.9230769230769231No Hit
CTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTG11.9230769230769231No Hit
GGGTTAAAGTTGAAAAGGAGGCCAAAGGCCTGGCCACCCCCCCCGCGGCC11.9230769230769231No Hit
TGGACTGGTTGTTGCCACCATCTGCCTATGAGACCGGTGCTGGGAGTCAG11.9230769230769231No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA11.9230769230769231No Hit
CCAACAATGGTGAGGATGCTACATCTGGTCTAACTCACATAATGATTTGG11.9230769230769231No Hit
CCTTTACTGCTCAGTCCAGACCCTGGGTATAAAATAGTTGGTCCATCATA11.9230769230769231No Hit
CAGTATGTGTGTGCATGTGTATGGATCTCTCAATCTTATTTTATTCACTA11.9230769230769231No Hit
ACTTTATAAGGACTACTTCTTATACCTGAAGTGAGGGCATATGAATTTGA11.9230769230769231No Hit
ATTATACTCCATAGGGTTTGTCATTGTTCATTTCTCTCCGGAACCATTTC11.9230769230769231No Hit
TGTCATGGGAGGGACCTGGTGGATGGTAATTGATCACTGGGGGCAGTCTC11.9230769230769231No Hit
AAATAGGATGGGGGCTGTAAACACTGAATTGGCATTTGGCCTTGTATGTG11.9230769230769231No Hit
GAGCGAAGCAGGGGGTTGGCTCATAGCTATTGTAGAGTTAATTCTCAGTT11.9230769230769231No Hit
GATTTGAAATAATCGAGGGGAGAGACAGAACAATGATTGAAGCCGAGTCC11.9230769230769231No Hit
CTCACTCCTGTAATCCCAGCACTTTGGGAGGCCGAGGCAGGTGGATCACG11.9230769230769231No Hit
CTGCTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAATAGGATGGGG11.9230769230769231No Hit
ATGTTAATTTTCAACTTAAGATGTGGAGAGATTTTAACTTGGATCAAGTG11.9230769230769231No Hit
GCTTCACCTCCTTTGCATCTACTCTAGGACCAAATTAATTTCCCAAGTTC11.9230769230769231No Hit
CTCGAAGTCAATTCTGGCATCAATCCGGGCCCTAGACACCATGGCCTCCC11.9230769230769231No Hit
CTTTTAGTTTGGACAATTTCTGTGAAATCATGTTTTTGTTGCACATGATG11.9230769230769231No Hit
TGTTGATTCCCACTAACTTGCAGGTCCTGTAGAATCTATCCACTCCTGCT11.9230769230769231No Hit
GGTCTCCACACCTGCTACACGATACATAACTGACATGACTATTGAGGAAT11.9230769230769231No Hit
ACTATAAGTCAAAGCTCACAGTACTACTTGCTTTGTGAGGTTTTTATAAA11.9230769230769231No Hit
AGCAGGTGGTGGCTGTGGGATGAGAAGGGCAAGGAGGAGGTGCTGGTAAT11.9230769230769231No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT11.9230769230769231No Hit
GTTATGTATCGCGAAGCAGGTGTGGAGACCATGGTCATTTTAAGTGCCTC11.9230769230769231No Hit
GAACTGAGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAGAGGTC11.9230769230769231No Hit
TAATAATACATTCCCACGTTGGTCTCGAACTCTCAAAAACCGATCAATGC11.9230769230769231No Hit
GACTTAAGTAATGGGGGCTGTCTGTGAAGCTTTGCAGCTGTACAGCCCAG11.9230769230769231No Hit
GGTGCAGTGGCTCATGCCTGTAATCCCAGCACTTCAGGAGGCCAAGGCGG11.9230769230769231No Hit
TTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTGAAGATGTC11.9230769230769231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTTG50.0145.09
CTTCACC50.0145.02
TTCACCT50.0145.03
CTCCTTT50.0145.08
ACCACTT50.0145.0145
CCTCCTT50.0145.07
CACCTCC50.0145.05
ACCTCCT50.0145.06
TCACCTC50.0145.04
GCTTCAC50.0145.01