FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971976
Sequences flagged as poor quality0
Sequence length151
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10830.11142250425936442No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA20200.068.9541
GAGCGAA6250.053.3933831
GCAAAAG26100.051.936833
CGAAAGC8150.051.5875363
CTTTCGC3700.050.954212
CAAAAGC26300.050.7154
GCGAAAG8300.049.797232
AGCAAAA29350.046.4470562
TCGCTCC4050.044.7465255
AAGCAGG38400.042.094537
AAAGCAG39200.041.7901956
GCTTTCG4550.041.454491
AGCGAAA9650.040.595331
GCAGTCG1252.7230635E-740.591966
AAAAGCA34850.038.896745
CGCTCCC5250.034.5169756
AGTAGAA16900.033.9117361
TTCGCTC5400.033.5598954
GCGAAGC900.00363078632.21753
TAGAAAC17650.032.034963
Produced by FastQC (version 0.11.5)