FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5659623
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT92490.16342077908722893No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA84960.15011600596011432No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT77240.13647552142607378No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC74190.13108646989384273No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC69530.12285270591345042No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG69090.12207526897109577No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA65470.11567908321808715No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63920.11294038489842875No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG58240.10290438073348702No Hit
TCCTTACTCTGGGTCTAGATCCGATATTCGGGATTACAGCTTGTTGGCTT57530.10164988021286932No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC57360.10134950684877773No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC27050.059.7649043
AGTAGAA36250.058.650451
AGCGAAA33750.046.869951
GCAAAAG113700.046.6723063
CGCCGTT2850.045.813503145
AAGCAGG154000.039.9151467
AGGCTCG4800.039.29144145
GCGAAAG40850.038.877892
CAGTACG16350.038.126939
GAGCGAA23150.037.2999081
CAAAAGC153050.036.6107644
GAGCAAA157650.035.3951841
TAGAAAC72100.035.0021442
AGCAGGT78600.033.3839048
TGACGCG5300.032.847416145
CGACTCG1601.8714836E-631.735394145
GTAGAAA82650.031.6061361
CCGTATA5300.031.478773145
AGGGTGT28650.030.6145026
CCAACTC47350.030.1616383
Produced by FastQC (version 0.11.5)