FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5236436
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC144990.27688679857826964No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC140930.2691334335032453No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT139960.26728102854689717No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG132000.2520798497298544No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA122320.2335939940829984No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA100190.1913324253366221No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA90850.1734958662724036No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT89350.17063132252547342No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA87290.16669734911302267No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC87030.1662008281968881No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC84480.16133110382710683No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT82250.15707248212333733No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT74680.14261608468049644No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC73400.14017167401644937No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA71860.13723074243626773No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTT64970.12407293815870184No Hit
GAGCAAAAGCAGGGAGCTTGAAAAGGAGATGGTATTGATTGCAAACAGGA62670.11968063774674224No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC62250.1188785654976018No Hit
TCCTAGTTCCAGTACTGGTTTAAGAGATGATCTTCTTGAAAATTTGCAGA56360.10763045705132268No Hit
CTCCTGTTCCACTTCAAACAGCAGTTGTAATGCTTGCATGAATGTTATTT54830.10470862242945393No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT54660.10438397413813516No Hit
TGATAACACAGTTCGAGTCTCTAAAAATCTACAGAGATTCGCTTGGAGAA54300.10369648363887193No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG54260.10362009580562045No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA27150.080.966551
GCAAAAG143700.080.215423
GCGAAAG49850.067.783722
GAGCAAA176350.066.152071
AGCGAAA51000.065.863331
CGAAAGC51750.065.4220353
CAAAAGC183450.064.0489964
AGCAAAA182750.063.286042
AGTAGAA57850.060.823481
GACGCGG10400.056.4539769
GCAGGGT61700.056.272119
AGACGCG10700.055.5485768
CGAGTCG3650.053.658867145
AGGGTGT38000.052.074126
TAGAAAC91300.051.8617362
AAAAGCA241350.048.9539035
AGCAGGG142550.048.0515568
AAGCAGG297400.045.820497
ACAAGGG81000.041.7974133
AGGCTCG6600.040.665714145
Produced by FastQC (version 0.11.5)