FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4639639
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA109050.069.334161
AGCGAAA52650.066.8418661
GCGAAAG53150.066.1702352
GCAAAAG143500.052.083463
CGAAAGC70300.050.6313253
GAGCGAA30050.050.4669461
AGCAAAA149800.049.6176532
CAAAAGC161900.046.8785554
AAAAGCA200950.037.9839975
AAGCAGG235000.035.6886867
AAAGCAG256300.035.0419046
AGTAGAA71650.031.4955651
GCCGACC11700.026.658632145
GTAGAAA91750.026.4146961
GAAAGCA139450.026.2510834
AGCAGGG140850.024.5995248
TAGAAAC96700.024.5889783
AGCAGGC71650.022.6614388
TGACGCG6450.021.367277145
AACAAGG133550.021.3389382
Produced by FastQC (version 0.11.5)