FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAACTTCCGAAGGGACCTCCTTGTTATCAGCGGTCCAAATTCCTCCTG23.225806451612903No Hit
TGTCTTTATGCACTTGATCGCATCATATAATGGGATCCCTTCTCATTCGT11.6129032258064515No Hit
CAAGTATCTCGTCCAGAAGTTATCGTAAAAGAAATCTATGGTGTGAAATG11.6129032258064515No Hit
CGACAGTCCTCATCAGATCCTTGATGGAAAAAACTGCACACTAATAGATG11.6129032258064515No Hit
GTTAAATATTTCTCTTGTTTGAGCAGTTTAAAACACTTTTTTTGTAGAAT11.6129032258064515No Hit
CTCCATGGAGAACAGTGAATCCTGCCCTAGTCCTTGATGGTGATGTCAGC11.6129032258064515No Hit
GTTCACTTGATCCAGCCATTTGCTCCATAGCCTTAGCTGTAGTGCTGGCC11.6129032258064515No Hit
TCGTCTGAGAGCTCGAAGACTCCCCTCCCCCGGAATGACACTTCTTCTGG11.6129032258064515No Hit
GTTCACTTGATCCAGCCATTTGCTACATAGCCTTAGCTGTATTGCTGGCC11.6129032258064515No Hit
TCTATCGACTCTTCAAACACGGCCTTAAAAGAGGCCCTTCTACGGAAGGA11.6129032258064515No Hit
AGCAAGCATTGACCTGAAGTATTTCAATGAATCAACAAGGAAGAAAATTG11.6129032258064515No Hit
GTTTTAACCTTTCGACTTTGTCGAAATAAGTCTTGTACACTTTTGGGTAA11.6129032258064515No Hit
ATGTGCCAGAGTTGATTTTAGGTATAAGGATATCCACTGACAAGATACGT11.6129032258064515No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC11.6129032258064515No Hit
CCTTTTCAATCTGATGGAATTTCTCGTTGGTTTTCCCGATCAATCTATTC11.6129032258064515No Hit
TTTCCAGGTAGATTGCTTTCTTTGGCATATCCGGAAACAAGTTGTAGACC11.6129032258064515No Hit
AGCGAAAGCAGGTCACTGCTGCCGCTGGCAGAAGCAAGTTACTGAGCACA11.6129032258064515No Hit
TCATTCCAGGGCTCAATGATGCTGTGCCATCTATTAGAAGAGGCCTTATT11.6129032258064515No Hit
GAAGGCAATGGTGAGATTTCGCCAACAATTGCTCCCTCTTCGGTGAAAGC11.6129032258064515No Hit
AAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGGCGTCC11.6129032258064515No Hit
CTATCACTCCGACAGATGCAGTTGAAGCTCTTCCAATCCTACACATCGAT11.6129032258064515No Hit
GTAGAAACAAGGGTATTTTTTTCATTAATTGTCATACTCTTCTGCATTGT11.6129032258064515No Hit
GCTCTGAAGAGGGGCCAGACTTGGGGAATTTGTGGGTGACCACTGGCCTT11.6129032258064515No Hit
TCTTTGTACAGAAAACGTAGGTTGCACACTGGTTTGGCCTGCGGAGGCCC11.6129032258064515No Hit
CTAATCAACAGAGGGCCTCCGCAGGCCAAACCAGTGTGCAACCTACGTTT11.6129032258064515No Hit
GAAATATCATCTAAGAGGATATGGAAATCACAAAAAAATTGGCAAAAGTA11.6129032258064515No Hit
ATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTCAAC11.6129032258064515No Hit
TATAAGAACATTGGCCTTGAAATTTGGCCTATGGCACTTCTTAGTAACAT11.6129032258064515No Hit
TTGTCAAATTGTTCATTGTTTGGCATAGTCACGTTCAATGCTGCGTATTT11.6129032258064515No Hit
GCATCATTCAAATTGGAATGCCAAATCATTATGTGAGTTAGACCAGATGT11.6129032258064515No Hit
TCCGTCGGGAAGATGATTGATGGAATTGGGAGATTCTACATCCAAATGTG11.6129032258064515No Hit
GTGTCGACCCAACTGACTCACCAGATAAATGGACTGTTTCAGGACGTGGA11.6129032258064515No Hit
AGGCAATACTCTCGGTCTAGACATCAAAGCAGCCACCCATGTTGGAAAGC11.6129032258064515No Hit
TTGTCTCGATAAGCATTGACAAGTGCAATTCTCCACGTCCTGAAACAGTC11.6129032258064515No Hit
CATTGGGATCTTGCACTTGATATTGTGGATTCTTGATCGTCTTTTTTTCA11.6129032258064515No Hit
GGATGGAGGCGGAGGTTGCAGTGAGCCGAGATCACACCATTGTACTCCAG11.6129032258064515No Hit
CAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACAATT11.6129032258064515No Hit
GGTTGATTCATTGTGATGTATGTAATCATAGCCAAAAACATTCGAGGGTT11.6129032258064515No Hit
GTTCAAATGGTTCGCATTTAACACCGTCGATTTCTTTTACGATAACCTCT11.6129032258064515No Hit
GAATAAGCATCTATTGGACAATAGTAAAACCGGGAGACATACTTTTGATT11.6129032258064515No Hit
ATACTCTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATAT11.6129032258064515No Hit
ATAACACAGTTCGAGTCTCTAAAAATCTACAGAGATTCGCTTGGAGAAGC11.6129032258064515No Hit
CTCCATGATTGCCTGGTCCATTCTGATGCAAAGAGGTCCTTCCATTTTCT11.6129032258064515No Hit
CAATTGCTCTTTGAGCAGCTGATTGAAATTTTCCTTTAAGAATGATGCAC11.6129032258064515No Hit
ATTCTACATCCAAATGTGCACTGAACTTAAACTCAGTGATCATGAAGGGC11.6129032258064515No Hit
AAATATGAGGTCTTCGATCTCAGCGTTTCCTGGGTTCCGACTTTCTCTCA11.6129032258064515No Hit
GTCCCACTGTGCCTGTAACCTCCTGTCTTGCTAATGTTCATGGCCCCTGC11.6129032258064515No Hit
CTGTTCTAGATAATGAGTGTTCAGTGGGAAGCAAAGTAGACAGAGTTTAG11.6129032258064515No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT11.6129032258064515No Hit
GTTCCAGTACTGGTTTAAGAGATGATCTTCTTGAAAATTTGCAGACCTAT11.6129032258064515No Hit
ATGTAAAGGCATCTTAGCCAAGATTTAGGATGGCAGACTCTGTCATGATT11.6129032258064515No Hit
GGACTGCAGCGTAGACGCTTTGTCAAAAATGCCCTCAATGGGAATGGAGA11.6129032258064515No Hit
GAGCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCT11.6129032258064515No Hit
GAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTGCCTGGC11.6129032258064515No Hit
TACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAAGAGTCGAT11.6129032258064515No Hit
ATTGCTGCTTGAGTGCTTTTGAGATCTGCTGCTTGTCCTCTTCCCTCAGA11.6129032258064515No Hit
TCTGTATACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCAGG11.6129032258064515No Hit
GGTTACTTCAAAATACGAAGTGGGAAAAGCTCAATAATGAGATCAGATGC11.6129032258064515No Hit
ATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTTGACATCCT11.6129032258064515No Hit
AAATTCCTCCTGATGGATGCTTTAAAATTGAGCATTGAAGACCCAAGTCA11.6129032258064515No Hit
CCTATCAATGGATCATCAGAAATTGGGAAGCTGTCAAAATTCAATGGTCT11.6129032258064515No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTCCT50.0145.05
AAATTCC50.0145.01
CTCCTGA50.0145.07
AATTCCT50.0145.02
CCTGATG50.0145.09
TTCCTCC50.0145.04
CCTCCTG50.0145.06
ATTCCTC50.0145.03
TCCTGAT50.0145.08
GGAATAA50.0145.0145
Produced by FastQC (version 0.11.5)