FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6871948
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCACACCATCGATTTCTTTTACGATAACTTCTGGACGAGATACTTG157710.22949824416599193No Hit
CTCCAACAGACGCGGTTGAAGCTCTTCCAATCCTACACATCGATGAGCCA138030.20086007635680597No Hit
GAGCAAAAGCAGGAGAAGCGAATGTGTATCGTTTGGCAGAAGTTTCTGTA134800.19615980796129423No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG121630.17699493651581763No Hit
AAACAAGGATTTGCAAAAAAGGCTCCTTTTTTGTGGATTTTCCAAGCGGC115350.16785633418646356No Hit
GAGCAAAAGCAGGTGTGTAGTTAAAACTAATACTCGGTGTCATCATATGT109900.1599255407636961No Hit
GAAATATACATTCCCATACCCACCAGAGGCAATTACTACAGCATGTGCAG92590.13473617669982368No Hit
GTGTAGCACAAGGGGTTCCTTTTGGTCGTGAATATGGCGGTCAGTTGGAT90080.1310836461509895No Hit
GTTGAATATCCAATCAAACCACGCGCTGGAACAAGGAAGACCAAACGAGT84700.12325471612998236No Hit
TTCCCATACCCACCAGAGGCAATTACTACAGCATGTGCAGAATGTCTCTC81530.11864175922169376No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74170.10793155012232339No Hit
ATATAATGGTACAAACGGGACTTAATTTCCGTAACGATGTGAATACCACA73590.10708753907916649No Hit
CATTTCACACCATCGATTTCTTTTACAATAACCTCTGGACGAGATACTTG72270.10516668636025768No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA71830.1045264021206214No Hit
ATTCTAAAGTGAATAATTATAGCATACAATGGATACACACTCAAGATGCC71240.10366783916292731No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA42300.098.108681
GAGCAAA205900.091.333571
CAAAAGC282700.065.693524
AGCAAAA303550.061.2017022
GCGAAAG99300.061.0480352
GACGCGG50550.060.6558469
AGACGCG51250.060.2516828
GCAAAAG308400.060.1054763
AAGCAGG371800.059.697427
CGAAAGC101900.059.54553
AAAAGCA333350.056.1020855
AGCGAAA109300.055.2938231
AAAGCAG426000.053.2595676
TCACACC84400.050.4144945
TTCACAC86200.049.7833024
AGCAGGT132000.048.8730558
ACCATCG86550.047.4864239
AGCAGGG208100.046.3965388
TAGAAAC91850.045.6313482
GCAGGGT74750.043.9279029
Produced by FastQC (version 0.11.5)