FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n02_HK05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n02_HK05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6593422
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG69900.10601475227886219No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA86750.077.037541
GCGAAAG88250.076.012592
CGAAAGC90350.074.382663
GAGCGAA36000.067.7250061
GAGCAAA134700.064.158831
CAAAAGC197400.043.4054574
GCAAAAG198600.043.257283
AGCAAAA208450.041.0524022
AAAAGCA261600.033.3625645
AAAGCAG337850.032.6553886
AAGCAGG324250.032.392367
GAAAGCA228950.030.3635084
AGCAGGG212900.023.6965168
TACCCCG25450.023.0710815
CCGCTCA29400.020.7101339
TAGCGAC8950.018.628544
GTATAGG32650.017.779481
CCCGCTC38350.016.254898
AGCAGGC111500.016.2523468
CCGACGC18300.015.855843145
Produced by FastQC (version 0.11.5)